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Exploring the Efficacy of Bioaugmentation Strategy in Microbial Degradation of Chloroaniline

1Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology, Enviro Technology Limited, Gujarat, India


Journal of Applied & Environmental Microbiology. 2014, Vol. 2 No. 4, 185-193
DOI: 10.12691/jaem-2-4-12
Copyright © 2014 Science and Education Publishing

Cite this paper:
M. Shah. Exploring the Efficacy of Bioaugmentation Strategy in Microbial Degradation of Chloroaniline. Journal of Applied & Environmental Microbiology. 2014; 2(4):185-193. doi: 10.12691/jaem-2-4-12.

Correspondence to: M.  Shah, Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology, Enviro Technology Limited, Gujarat, India. Email: shahmp@uniphos.com

Abstract

Pseudomonas spp. was isolated from activated-sludge and found to be able to mineralize 3-chloroaniline (3-CA). This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation in activated-sludge. To monitor its survival, the strain was chromosomally marked with the gfp gene and designated Agfp. After inoculation into a lab-scale semi continuous activated-sludge (SCAS) system, the inoculated strain maintained itself in the sludge at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during two weeks, while no degradation at all occurred in the non-inoculated control reactors. Upon further operation of the SCAS system, only 50 % 3-CA removal was observed. Denaturing Gradient Gel Electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated-sludge. The DGGE patterns of the non inoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.

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