Kiyindou-Soungadia L.F.C¹, Wossolo Lingomo B.S^{1, 2}, Lebonguy A.A³, Etou Ossibi A.W^{1, 4,}

For centuries, various pathologies have been treated with traditional medicines derived from medicinal plants. Such remedies must meet three requirements: safety, therapeutic efficacy, and quality. The objective of this study was to assess the anti-ulcer effect, microbiological and physicochemical quality, and phytochemical profile of a revealed medicinal recipe (RMR). Two models of gastroduodenal ulcer induction using two ulcerogenic agents were employed to evaluate the anti-ulcer effect in laboratory rodents. Classical methods were applied for microbiological and physicochemical quality assessment, following standardized AFNOR procedures, while phytochemical profiling was carried out using tube coloration reactions. The results showed that the recipe protects the gastric mucosa against ulcers induced by hydrochloric acid–ethanol mixture and ethanol in the presence of diclofenac. The recipe was free of pathogenic flora (Salmonella and Staphylococcus aureus) as well as total and fecal coliforms. The contamination levels of total aerobic mesophilic flora were 2.2×10⁶ CFU/mL and 10⁴ CFU/mL for yeasts and molds, compared with the standards of 10³ and 10² CFU/mL, respectively. Moreover, the pH, electrical conductivity, total dissolved solids, and solvent content were 4.61, 3.625 mS/cm, 1835 ppm, and 20.04%, respectively. Phytochemical screening revealed the presence of tannins, alkaloids, flavonoids, anthraquinones, oses and holosides, mucilages, reducing sugars, and the absence of saponosides. In conclusion, the revealed medicinal recipe is a mucosal protector of good microbiological and physicochemical quality and is rich in secondary metabolites.

Ulcers, microbiological quality, phytochemistry, revealed medicinal recipe