Objectives: This study aimed to determine the antibacterial effectiveness of adding zirconia (ZrO2NPs), silver (AgNPs), and titanium dioxide (TiO2NP) nanoparticles in various concentrations to 3D-printed denture resin against C. Albicans, S. pyogenes, and S. aureus. Methods: Power analysis was used to count the in vitro samples. Using World Health Organization calculations, a research power of 80%, a significance level of 5%, and an error margin of 5% were determined. The antimicrobial efficacy of 150 disk-shaped specimens with a diameter of 15 X 2 mm of unmodified (n= 15) and modified (n= 135) 3D-printed denture resin specimens after the addition of silanated ZrO2NPs, AgNPs, or TiO2NPs (n= 45) in varying concentrations (n= 15) of 0.5%, 1%, and 1.5% were compared using three oral bacteria (Streptococci pyogenes, Staphcoccous Aureus, and Candida Albicans) as test subjects. Colony forming units (CFUs) were counted as part of the antimicrobial test. The CFUs count was statistically analyzed using the one-way ANOVA test. Patients subjected to antimicrobial test. Results: When the three tested nanoparticles (ZrO2NPs, AgNPs, and TiO2NPs) were added, the bacterial count significantly decreased as compared to the unmodified 3D-printed resin, according to the results. Additionally, the findings showed that as the concentration of the studied nanoparticles increased, so did their antibacterial activity. At 1.5% and 1%20 concentrations, the AgNPs' antibacterial activity was negligible. However, the in vitro study's findings showed that, in terms of the kinds of microorganisms studied, there were no appreciable variations between the three tested nanoparticles.Conclusions: The antibacterial activity of the resin used in 3D printing was significantly affected by the inclusion of ZrO2 NPs, AgNPs, and TiO2NPs.

Antibacterial activity, Denture Resin, 3D Printed, Nanoparticles