Yousif A. Elhassaneen^1,, Amal N. Zaki¹, Basma G. Helmy¹, Asmaa I. Bayomi²

The present study aims to investigate the effect of Sargassum subrepandum powder feeding intervention on biochemical, molecular and histological liver damage induced by benzo[a]pyrene in rats. Sargassum subrepandum powder showed a high nutritional composition through its high of essential nutrients (carbohydrates, fiber, protein and ash). It also contains many of the following bioactive constituents: polyphenols, Anthocyanin's, Carotenoids, Kaempherol, polysaccharides, terpenoids, triterpenoids, flavonoids, carotenoids, tannins, and dietary fiber, which resulted in high biological activates including antioxidant and peroxyl radical (ROO-)-scavenging activities and inhibition of lipid peroxidation. For biological experiments, rats (n=36), were randomly assigned to six groups of 6 rats per each. Group 1 served as normal control. Groups 2 to 6 were injected intraperitoneally with B[a]P for 14 days and group (2) acted as a model control, while groups (3-6) received Sargassum subrepandum powder at concentrations of 1, 2, 3, and 4 g/100g diet for 28 days each, respectively. B[a]P-injected rats exhibiting significantly (p≤0.05) increased levels of AST (117.02%), ALT (120.24%) and ALP (115.75%) as well as decreased levels of serum albumin (45.67%) compared to the normal group. Also, data indicated that B[a]P induced significantly (p≤0.05) decreasing in liver GSH level by the ratio of -40.42% whereas, the liver MDA concentration was exhibited the opposite direction, increased by the rate of 195.54% compared to the normal group. Intervention with brown algae (Sargassum subrepandum powder (1- 4.0 g/100g diet) for 28 days significantly (p≤0.05) decreased the levels of liver function enzymes activities (AAST, ALT and ALP) and liver level of MDA, and increased serum albumin and liver level of GSH by different rates compared to the model group. Also, improvement in both molecular and histological parameters was recorded. The rate of improving in all of these parameters exhibited a dose-dependent manner with the Sargassum subrepandum powder intervention. The results of this study suggest that treatment with Sargassum subrepandum powder in the tested concentrations proved beneficial on manipulation of the liver biochemical, molecular and histological injuries induced by B[a]P.

chemical composition, bioactive compounds, liver functions, glutathione, malonaldehyde, electrophoretic pattern of nucleic acid, apoptosis