Kouakou Myrielle Fatoumata1, 2,
Toty Abalé Anatole1,
,
Gadou Victoire3,
M’Bengue Gbonon Valérie1,
Konan Kouadio Fernique1,
Guédé Kipré Bertin1,
Ouattara Mohamed Baguy1,
Tiécoura Kouakou2,
Guessennd Nathalie1,
Dosso Mireille1 1Pasteur Institute of Côte d’Ivoire, 01 BP 490 Abidjan 01 Côte d’Ivoire
2Genetics Laboratory, UFR Biosciences, Felix Houphouët-Boigny University, 01 BP V 34 Abidjan 01, Côte d’Ivoire
3Department of Biochemistry-Genetics, UPR Microbiology, UFR of Biological Sciences, Peleforo Gon Coulibaly University, BP 1328 Korhogo, Côte d’Ivoire
American Journal of Microbiological Research.
2023,
Vol. 11 No. 3, 79-82
DOI: 10.12691/ajmr-11-3-3
Copyright © 2023 Science and Education PublishingCite this paper: Kouakou Myrielle Fatoumata, Toty Abalé Anatole, Gadou Victoire, M’Bengue Gbonon Valérie, Konan Kouadio Fernique, Guédé Kipré Bertin, Ouattara Mohamed Baguy, Tiécoura Kouakou, Guessennd Nathalie, Dosso Mireille. Detection of
qnr Genes That Mediate Fluoroquinolone Resistance in Gram-Negative Bacilli in Abidjan, Côte d'Ivoire.
American Journal of Microbiological Research. 2023; 11(3):79-82. doi: 10.12691/ajmr-11-3-3.
Correspondence to: Toty Abalé Anatole, Pasteur Institute of Côte d’Ivoire, 01 BP 490 Abidjan 01 Côte d’Ivoire. Email:
totyabale@yahoo.frAbstract
The public health threat of antimicrobial resistance remains a major challenge that keeps expanding as a result of the continuous dissemination of mobile genetic elements that mediate resistance among bacteria species. This study aimed to detect genetic determinants (qnrA, qnrB, qnrC, qnrD, qnrS, and qnrVC genes) that mediate fluoroquinolone resistance in Gram-negative bacilli in Côte d'Ivoire. A total of 30 strains were characterized by biochemical tests and their identity was confirmed by MALDI-TOF mass spectrometry. Antimicrobial susceptibility testing was performed by the agar diffusion method and the detection of qnr genes by polymerase chain reaction (PCR). Fifteen (57.7%) of the Enterobacteriaceae were extended-spectrum beta-lactamase (ESBL) producers. Susceptibility testing revealed resistance rates of 76.7%, 93.3%, 83.3%, and 65.2% for nalidixic acid, norfloxacin, ofloxacin, and levofloxacin respectively. Detection of fluoroquinolone resistance genes showed the presence of qnrD (36.7%), qnrB (36.7%), qnrS (13.3%), and qnrA (6.7%). This study is the first to demonstrate the presence of the qnrD gene in some Gram-negative bacilli in Côte d'Ivoire.
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