Article citationsMore >>

Kokalis-Burelle, N., Backman, P.A., Rodriguez-Kabana, R., Ploper, L.D., “Potential for biological control of early leaf spot of peanut using Bacillus cereus and chitin as foliar amendments”, Biol. Control, 2: 321-328. 1992.

has been cited by the following article:

Article

In Vitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil

1Department of Plant Protection, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan

2Department of Plant Protection, Faculty of Agriculture, University of Khartoum, Khartoum, Shambat, Sudan

3Department of Crop Sciences, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan


World Journal of Agricultural Research. 2014, Vol. 2 No. 2, 47-50
DOI: 10.12691/wjar-2-2-3
Copyright © 2014 Science and Education Publishing

Cite this paper:
Saifeldeen A. Abdalla, Soad A. A. Algam, Elshiekh A. Ibrahim, Ahmed M. El Naim. In Vitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil. World Journal of Agricultural Research. 2014; 2(2):47-50. doi: 10.12691/wjar-2-2-3.

Correspondence to: Ahmed  M. El Naim, Department of Crop Sciences, Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied, Sudan. Email: naim17amn@yahoo.com

Abstract

Rhizosphere bacteria are one of the most potential biological control agents in the plant disease protection. Bacillus species as a group offer several advantages over other bacteria for protection against pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. Five soil samples from tomato rhizosphere were collected from shambat area, Khartoum State, Sudan. Bacillus isolates were isolated from the rhizosphere of tomato to use as natural bio-control agents. They were screened for antagonism in vitro against Alternaria alternata causal agents of early blight disease of tomato. Serial dilution technique was adopted for the isolation of Bacillus species. Only 27 out of 45 Bacillus isolates showed antagonistic properties. Four out of the 27 isolates showed antagonism (Bacillus B25, B35, B41, B45) were identified to the species level by bacteriological assay (morphological and biochemical tests).

Keywords