Deniel Caiyl J. Remollo^1,, Brian Jerome I. Ortizano¹, Erika Xenia Gem M. Abunda¹, Aleshxandra B. Leyros¹, Ian Jay P. Saldo¹, Mary Jade Peñafiel-Dandoy¹

Antibacterial resistance has become a worldwide public health concern, prompting the search for alternative antibacterial agents. Novel antibacterial agents have already been studied; however, their cytotoxicity must be evaluated first to guarantee their safety on normal cell lines. This study examines the cytotoxicity and antibacterial activity of jackfruit (Artocarpus heterophyllus) rind extracts on Staphylococcus aureus and Salmonella spp. This study utilized Brine Shrimp Lethality Assay for the cytotoxicity test while the Kirby-Bauer method for the antibacterial test. The results of the study indicated that the A. heterophyllus rind extract had an LC50 value of 559.4, which is considered toxic according to Meyer's criteria of toxicity and is categorized as low toxic according to Clarkson's criteria of toxicity. The study also found that the lethality of the rind extract was concentration dependent. Furthermore, the results showed that the rind extracts of A. heterophyllus did not successfully prevent the growth of S. aureus and Salmonella spp., as all samples of the rind extract yielded a diameter of 0 mm. Further research on the cytotoxicity and antibacterial activity of jackfruit rinds using more concentrations and various solvents for the extraction method, as well as for the diluent, is recommended.

antibacterial activity, brine shrimp lethality assay, cytotoxicity, gram-positive, gram-negative, jackfruit rind extract