Zhaoming Liu^1,, Shengying Wang¹, Mingliang Ge²

In search of novel artificial mimic enzymes with catalytic properties, new nitrogen-containing carbon nanomaterials were designed and fabricated using polypyrrole as a precursor and magadiite, a naturally occurring layered clay, as a template. X-ray diffraction spectroscopy (XRD), Fourier infrared transform spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and field emission scanning electron microscopy (FESEM) were used to describe the structure, morphology, and functional components of carbon nanomaterials. The reducing substrate used was 3,3,5,5-tetramethylbenzidine (TMB), which could be oxidized and discolored by the nanozyme in the presence of H₂O₂. Initial simulated enzymatic activity probes demonstrated that the nanocarbon material containing nitrogen had a strong affinity for the chromogenic substrates TMB and hydrogen peroxide. The Michaelis constant for the substrate TMB is 0.452 mM, which is comparable to that of the natural peroxidase HRP, whereas the Michaelis constant for the substrate hydrogen peroxide is 1.89 mM, which is approximately 50% lower than that of HRP, indicating that its affinity for the substrate H₂O₂ is greater than that of HRP. The nitrogen-containing carbon nanomaterials' enzyme-like activity maintained high catalytic activity in extreme conditions such as strong acid and high temperature, giving them the potential for widespread use and stable storage.

nitrogen-containing nanocarbon, magadiite, peroxidase, H₂O₂