Anbazhagan Vijayakumar¹, Atmakuru Ramesh^1,

Malaria is one among the major public health problems with an estimated million cases worldwide every year. WHO recommends the use of insecticide-treated nets, particularly long-lasting insecticidal nets (LLIN), to reduce malarial deaths [1]. The treated bed nets have been factory incorporated and coated with long-lasting insecticidal Nets (LLINs) to protect people from malaria and also reduce incidence of malaria in a population [2]. Pyrethroid insecticides have been extensively used for the treatment of nets to protect against malaria and other vector-borne diseases. The new LLIN products are under the development and its requiring assessment of risks to humans. Which have been shown to be cost-effective to reduce malaria for children (< 5 years of age). Striving plans for their extensive use in worldwide for self-protection from mosquitoes [3], prevention from malaria and another severe disease. The health risks from the LLINs have not been sufficiently investigated and reported in the peer-reviewed scientific literature [4,5]. Here, we use a probabilistic risk assessment approach to estimate the risks when a child chewing the pesticide treated nets while sleeping under the bed nets [6]. The experiment was conducted in artificial biological fluid to determine the amount of potential active to be leaching into the biological fluid which is artificial saliva during overnight contact. The concentration of active ingredient content release proportion from the long-lasting insecticidal net into the artificial saliva was determined by using LC-MS/MS [7,8]. During the leaching experiment at predetermined intervals at 0.5, 2, 4, 8, 12, 16, and 24 hours after immersing in artificial saliva at 36±2°C.

LC-MS/MS, leaching, LLIN, pesticide, residue, household, malaria, MRM, risk of LLIN