1Department of Microbiology, Rayat Institute of Research and Development, Satara, Shivaji University, Kolhapur-416004, Maharashtra, India
2Department of Microbiology, Miraj Mahavidyalaya, Miraj - 416410, Maharashtra, India
3Department of Microbiology, Yashwantrao Chavan Institute of Science, and Rayat Institute of Research and Development, Satara - 415001, Maharashtra, India
Applied Ecology and Environmental Sciences.
2022,
Vol. 10 No. 4, 219-224
DOI: 10.12691/aees-10-4-6
Copyright © 2022 Science and Education PublishingCite this paper: Oliver Madhale, Vinay Chougule, Ujwala Mane. Isolation and Identification of Antimicrobial Actinomycetes from Non-Agricultural Soil.
Applied Ecology and Environmental Sciences. 2022; 10(4):219-224. doi: 10.12691/aees-10-4-6.
Correspondence to: Oliver Madhale, Department of Microbiology, Rayat Institute of Research and Development, Satara, Shivaji University, Kolhapur-416004, Maharashtra, India. Email:
oliver.paul.madhale@gmail.comAbstract
Potential Actinomycetes produced biologically significant secondary metabolites in abundance in non-agricultural farm. Actinomycetes were isolated from soil on a non-agricultural farm in Miraj, Sangli District, Maharashtra, with only six isolates chosen for this study. Streptomyces spp. isolates demonstrated strong antimicrobial activity against human pathogenic strains of Salmonella typhi, Salmonella paratyphi A, and Salmonella paratyphi B pathogens. The isolate INA-53 had excellent antimicrobial activity against enteric fever pathogens zone of inhibition were observed at the highest level (16.0-23.0 mm). According to BLAST analysis, INA - 53 is related to Streptomyces parvulus Actinomycetes and the Actenobacteria class. As a result, the powerful INA - 53 isolate is extremely effective at producing bioactive compounds.
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