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Xu, Z.H., Bai, Y.L., Xu, X., Shi, J.S. and Tao, W.Y, “Production of alkali-tolerant cellulase-free xylanase by Pseudomonas sp. WLUNO24 with wheat bran as the main substrate”, World Journal of Microbiology and Biotechnology, 21: 575-581. 2005.

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Article

Production, Purification and Characterization of Xylanase Enzyme from Bacillus sp in Solid State Fermentation

1Department of Chemistry, Sree Siddaganga College of Arts, Science and Commerce, Tumakuru-572102, (Karnataka), India

2Department of Biotechnology, Azyme Biosciences Private Limited, Bengaluru-560069, (Karnataka), India


Applied Ecology and Environmental Sciences. 2021, Vol. 9 No. 7, 640-648
DOI: 10.12691/aees-9-7-3
Copyright © 2021 Science and Education Publishing

Cite this paper:
Bidare Shivayogiraju Shylesha, Mahesh Mariswamy, Shubharani Ramanath, Veerapura Narayanappa Yoganandamurthy. Production, Purification and Characterization of Xylanase Enzyme from Bacillus sp in Solid State Fermentation. Applied Ecology and Environmental Sciences. 2021; 9(7):640-648. doi: 10.12691/aees-9-7-3.

Correspondence to: Veerapura  Narayanappa Yoganandamurthy, Department of Biotechnology, Azyme Biosciences Private Limited, Bengaluru-560069, (Karnataka), India. Email: dryoganand16@gmail.com

Abstract

Xylanases are extensively applied in paper and pulp industries as well as during preparation of baked products to improve their quality. Additionally, it is also used in coffee, oil and starch industries in order to increase their nutritional values. Soil samples were collected near saw mills in various localities of Bangalore urban to isolate organisms for the production of xylanase using solid state fermentation. Six organisms were isolated using selective media based on their morphological characters. Among them one organism showed maximum production of xylanase enzyme identified as Bacillus sp based on their biochemical test and 16s RNA sequencing. Solid substrate fermentation was carried out using various agro wastes such as sugar cane bagasse, saw dust, paddy husk, wheat straw and orange peel powder. Sugarcane bagasse showed maximum production of enzyme compared to other substrates with different physical parameters such as pH 8, temperature at 35 °C after 72 hrs of incubation. Trace element such as Mg++ enhances the production of enzyme more than 22 % compared with other metal ions like Ca++, Mn++ and Fe++. After production, enzyme purified by using three step methods such ammonium sulphate precipitation, dialysis, ion exchange and gel filtration. Fold purification was increased up to 12 fold, yield 36 % and molecular weight of enzyme was 62 KDa determined using SDS PAGE.

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