1Department of Biochemistry, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation
2Laboratory of Biochemistry and Immunology. National University of Sciences, Technologies, Engineering and Mathematics. BP: 72 Natitingou, Republic of Benin
3Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Technology, University of Abomey-Calavi, Republic of Benin
4Research Unit in Applied Microbiology and Pharmacology of natural substances, Laboratory of Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi
5Department of Invertebrate Zoology and Functional Histology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russian Federation
6Clinic of allergic diseases of Kazan Research Institute of Epidemiology and Microbiology, Kazan, Russian Federation
7Kazan state medical Academy, Kazan, Russia Federation
American Journal of Biomedical Research.
2021,
Vol. 9 No. 1, 10-18
DOI: 10.12691/ajbr-9-1-3
Copyright © 2021 Science and Education PublishingCite this paper: Yulia V. Skibo, Cyrille A. Vodounon, Boris B. Legba, Sergey N. Abramov, Vladimir G. Evtugyn, Irina D. Reshetnikova, Zinaida I. Abramova. Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma.
American Journal of Biomedical Research. 2021; 9(1):10-18. doi: 10.12691/ajbr-9-1-3.
Correspondence to: Cyrille A. Vodounon, Laboratory of Biochemistry and Immunology. National University of Sciences, Technologies, Engineering and Mathematics. BP: 72 Natitingou, Republic of Benin. Email:
sweetiebj@yahoo.frAbstract
The increased interest in the process of autophagy in various physiological and pathological conditions is the focus of this study. Therefore, the aim was to assess the involvement of lymphocyte autophagy in the pathogenesis of bronchial asthma. Forty-five patients with mild persistent asthma, 45 patients with severe persistent asthma, and 45 normal healthy controls were involved in this study. Autophagy was evaluated based on the expression of microtubule-associated protein light chain 3 (LC3) by western blot, Fluorescence microscopy, and flow cytometry. Transmission Electron Microscopy was used to detect autophagosome. The results showed that autophagy was activated in T-cells of patients with mild and severe asthma compared to the normal healthy control. Stress conditions induced autophagy in T-cells of asthmatic patients with mild and severe form, but not in the control group. Dexamethasone treatment of T-cells stimulated apoptosis in mild asthma patients and in the control group but in the group with severe asthma, dexamethasone induced autophagy. Thus, autophagy could play an important role in the pathogenesis of asthma especially of severe asthma, and may contribute to the survival and the activation of T-lymphocyte in patients with severe asthma.
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