Azo dyes represent a major group of dyes causing environmental concern because of their colour, biorecalcitrance nature and potential toxicity to living beings. In the present study an attempt was made to examine the potential of Pseudomonas putida for decolorization of azo dye-Remazol Black B in batch reactor. The influence of different concentration of glucose, pH and temperature on decolorization was studied to find the optimum conditions required for maximum decolorization and degradation. pH 7.0 and 350 C were considered to be the optimum decolorizing conditions because in these conditions only the maximum decolorize was found. 5 g/L glucose present media showed the maximum decolorization. The bacterium exhibited a remarkable color removal capability over a wide range of dye concentration (50-200 mg/l), Colorless cells of P. putida and UV Visible spectroscopic analyses suggested that the decolorizing activity only through biodegradation not by inactive surface adsorption. The above results show the potential of this bacterial strain to be used in the biological treatment of textile effluent under optimum condition.

Pseudomonas, Decolorization, pH, temperature, Remazol Black-B