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Article

Drug Resistant Bacteria are Growing Menace in a University Hospital in Nepal

1Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal

2Department of Biochemistry, Kathmandu University School of Medical Science, Kathmandu University, Kavre, Nepal

3Department of Microbiology, Kathmandu Medical College, Kathmandu University, Kathmandu, Nepal


American Journal of Epidemiology and Infectious Disease. 2014, Vol. 2 No. 1, 19-23
DOI: 10.12691/ajeid-2-1-4
Copyright © 2014 Science and Education Publishing

Cite this paper:
Shyam Kumar Mishra, Bal Krishna Awal, Hari Prasad Kattel, Jyoti Acharya, Prabin Gyawali, Chandra Prakash Bhatt, Niranjan Prasad Shah, Keshab Parajuli, Basista Prasad Rijal, Bharat Mani Pokhrel. Drug Resistant Bacteria are Growing Menace in a University Hospital in Nepal. American Journal of Epidemiology and Infectious Disease. 2014; 2(1):19-23. doi: 10.12691/ajeid-2-1-4.

Correspondence to: Shyam  Kumar Mishra, Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal. Email: smishra7@hotmail.com

Abstract

Large amounts of antibiotics used for human therapy has resulted in the selection of pathogenic bacteria resistant to multiple antimicrobial drugs. This has created problems in the treatment of patients. So, this study was carried out to determine multidrug resistant (MDR) bacterial pathogens and their antibiogram in patients with clinically suspected pneumonia attending a tertiary care centre in central Nepal. Specimens representing lower respiratory tract were processed using standard protocol. Antibiotic susceptibility test was performed on bacterial pathogens by Kirby-Bauer disk diffusion method following Clinical and Laboratory Standards Institute guidelines. Fifty-four percent of the total bacterial isolates were MDR. Multidrug resistance was found in Klebsiella pneumoniae (23.4%), Pseudomonads (20.5%), Acinetobacter calcoaceticus baumannii complex (20.6%), Escherichia coli (11.6%), Staphylococcus aureus (9.1%) and others. Non-fermentative bacteria were more multidrug resistant (MDR) than Enterobacteriaceae (77.8% vs. 68.9%) whereas extended-spectrum beta-lactamase (ESBL) was considerably higher among Enterobacteriaceae (37.27% vs. 10.46%). Resistance was seen even against carbapenems. Only polymyxins were effective against multidrug resistant gram-negative bacterial isolates. This study shows an emergence of MDR bacterial pathogens at an alarmingly high level as the isolates were resistant to almost all antibiotics commonly used in our set-up. There must be prudent use of antibiotics to prevent the emergence of MDR bacterial isolates.

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