Article citationsMore >>

Mahshid, Sara, Chengcheng Li, Sahar Sadat Mahshid, Masoud Askari, Abolghasem Dolati, Lixia Yang, Shenglian Luo, and Qingyun Cai. "Sensitive determination of dopamine in the presence of uric acid and ascorbic acid using TiO2 nanotubes modified with Pd, Pt and Au nanoparticles," Analyst 136 (no.11): 2322-2329, 2011.

has been cited by the following article:

Article

Functionals Aspects and Simultaneous Detection of Dopamine, Ascorbic Acid and Uric Acid Using Chitosan-Catechol Graphene and Carbon Nanotube Modified Electrodes

1Bioengineering, George Mason University, Fairfax, USA

2Neuroscience, George Mason University, Fairfax, USA

3Chemistry and Biochemistry, George Mason University, Fairfax, USA

4Biomedical Engineering, Ben-Gurion University of the Negev, Sheva, Israel


Journal of Biomedical Engineering and Technology. 2019, Vol. 7 No. 1, 5-13
DOI: 10.12691/jbet-7-1-2
Copyright © 2019 Science and Education Publishing

Cite this paper:
Tjerignimin Adissa Silue, Valerie Lewitus, Abul Hussam, Hadar Ben-Yoav, Nathalia Peixoto. Functionals Aspects and Simultaneous Detection of Dopamine, Ascorbic Acid and Uric Acid Using Chitosan-Catechol Graphene and Carbon Nanotube Modified Electrodes. Journal of Biomedical Engineering and Technology. 2019; 7(1):5-13. doi: 10.12691/jbet-7-1-2.

Correspondence to: Tjerignimin  Adissa Silue, Bioengineering, George Mason University, Fairfax, USA. Email: tsilue@gmu.edu

Abstract

Dopamine functions as a neurotransmitter in the brain. The dysfunction of the dopaminergic system is the leading cause of numerous diseases such as Parkinson’s disease. Hence, it is important to find selective and sensitive detection methods for the early diagnosis of diseases related to the abnormal levels of dopamine. In this study, we show a new electrochemical sensing platform based on carbon nanotube (SWCNT) and a sheet of graphene (GRA). The novelty of our sensor is the coating of the substrates with chitosan-catechol (CC) by electrodeposition, enhancing the dopamine response by 70%. The dose-response for each set of electrodes (bare CNT, bare GRA, and coated CNT as well as coated GRA) was measured. Finally, the electrodes were tested in cerebrospinal fluid (artificial and human), for the detection of millimolar to nanomolar levels of dopamine. The electrodes exhibited high sensitivity (2.03mA mol·L-1, 1.45 mA mol·L-1, 0.0298 mA mol·L-1, and 0.0559 mA mol·L-1 for the modified CNT, bare CNT, modified GRA and bare GRA, respectively, for the oxidation of DA. The oxidation peak current was proportional to the concentration of DA in the range from 50×10-6 to 50x10-9 M (n= 6, r2 =0.98). The dopamine recovery in human CSF were, 49-78% and 65-65% with coated graphene and CNT electrodes, respectively. Our results indicate that the CC modified CNT electrodes achieved the best recovery, sensitivity, limit of detection, and selectivity compared to the uncoated CNT as well as the coated and uncoated graphene electrodes.

Keywords