1Faculty of Medical Laboratory Science, The National Ribat University
2Research Laboratory, Faculty of Medical Laboratory Science, The National Ribat University
3Faculty of Medical Laboratory Science, Karari University
4Department of Parasitology & Molecular Biology, Institute of Endemic Disease
American Journal of Microbiological Research.
2018,
Vol. 6 No. 4, 181-186
DOI: 10.12691/ajmr-6-4-6
Copyright © 2018 Science and Education PublishingCite this paper: Ibrahim H.S, Kafi S.K, Musa H.A, Karsani M.S, Mahadi A.M. Detection, Identification & Sequencing of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) among Sudanese Patients.
American Journal of Microbiological Research. 2018; 6(4):181-186. doi: 10.12691/ajmr-6-4-6.
Correspondence to: Ibrahim H.S, Faculty of Medical Laboratory Science, The National Ribat University. Email:
hibasiddig55@gmail.comAbstract
MERS-CoV virus is a newly emerged coronaviruses in KSA in 2012; followed by a lot of cases in the Middle East & other European, American & African countries. The goal of this study is to detect, identify & sequencing of MERS-CoV among Sudanese patients suffering from respiratory diseases by using the orf1a with upE gene for the virus detections. Phylogenetic analysis of upE gene of MERS-CoV was done using ViPR. MERS-CoV virus seems to be highly prevalent among Sudanese population especially among patients from Al-Shaab hospital than individuals from the International Khartoum Airport (86.8% & 12.1% vs 66.6% & 9.0%) respectively; this attributed to the fact that Al-Shaab hospital participants were symptomatic, having severe respiratory symptoms (possibly caused by coronaviruses). Phylogenetic analysis of the isolated viruses from Khartoum International Airport participant showed genetic similarity with KSA while Al-Shaab Teaching Hospital individuals showed genetic similarity with Thailand. Viral genomic were deposit in gene bank with those accession N.o: KY794400.1-KY794403.1 and MF133448-MF133457.
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