Andriambeloson Onja¹, Rabenirina Harilanto¹, Rajoelisoa Andriamalala¹, Ramaroson Luciano¹, Rasolomampianina Rado^1,

The ability of Actinomycetes strains to degrade pollutant matters and to reduce or eliminate pathogens microorganisms from domestic wastewater of an industrial site (oilfield of Tsimiroro-Madagascar) at the laboratory scale is demonstrated in the present work. Two most active Actinomycetes isolates (Streptomyces albidoflavus strain DSM 40455T and Streptomyces antibioticus strain NBRC 12838T) against test-pathogens were selected for the purification treatment. The analysis of physico-chemical (COD, BOD, pH, conductivity, color, TDS, nitrite, nitrate, phosphate and chloride rates) and microbiological parameters (sulphite reducing anaerobe, fecal coliforms, fecal Streptococcus and Escherichia coli rates) allowed to evaluate the quality of the wastewater. Physico-chemical results revealed that purified water is qualitatively improved view that 60.86% of TDS, 71.61% of its color, 25.55% of its chloride rate, 45.32% of its nitrate rate, 99.9% of its nitrite rate, 26.25% of its phosphate rate, 46.53% of its initial COD and 58.11% of its BOD were eliminated at the end of the treatment. Only, the conductivity increased compared with the guideline values for all treatment. The process improved also microbiological quality of the wastewater with total elimination of fecal Streptococcus and diminution of fecal coliforms, sulphite reducing anaerobe and Escherichia coli concentrations. The experiment proved that biological treatment using Actinomycetes strains is a promising, less expensive and simple technology for wastewater recycling ensuring thus their reuse for other activities.

wastewater, biological treatment, Actinomycetes, microbiological quality, physico-chemical quality