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Graham DA, Foster JC, Mawhinney KA, Elvander M, Adair BM, Merza M. Detection of IgM responses to bovine respiratory syncytial virus by indirect ELISA following experimental infection and reinfection of calves: abolition of false positive and false negative results by pre-treatment of sera with protein-G agarose. Vet Immunol Immunopathol 1999, 71(1): 41-51.

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Article

Bacterial Immunoglobulin (Ig)-Receptors: Past and Present Perspectives

1Department of Para-clinical Sciences, The University of the West Indies, Trinidad and Tobago

2Department of Basic Medical Sciences, The University of the West Indies, Kingston, Jamaica

3Department of Microbiology, The University of the West Indies, Kingston, Jamaica


American Journal of Microbiological Research. 2017, Vol. 5 No. 2, 44-50
DOI: 10.12691/ajmr-5-2-4
Copyright © 2017 Science and Education Publishing

Cite this paper:
Angel Justiz-Vaillant, Norma McFarlane-Anderson, Monica Smikle. Bacterial Immunoglobulin (Ig)-Receptors: Past and Present Perspectives. American Journal of Microbiological Research. 2017; 5(2):44-50. doi: 10.12691/ajmr-5-2-4.

Correspondence to: Angel  Justiz-Vaillant, Department of Para-clinical Sciences, The University of the West Indies, Trinidad and Tobago. Email: angel.vaillant@sta.uwi.edu

Abstract

The new contributions of this paper include the identification of bacterial Ig receptors as valuable reagents for the detection of Ig molecules in species of wild, domestic and laboratory animals. It is important to detect antibodies as markers of infection and zoonotic diseases. The techniques used to investigate the binding of the bacterial Ig receptors with immunoglobulins present in different specimens were established techniques such as ELISA and immunoblot analyses. In addition, the affinity chromatography allowed for the purification of immunoglobulins and their fragments. The use of commercially available conjugates of enzyme labelled proteins L, A, G and SpLA is discussed.

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