Muslimin Lucia^1,, Sri Rahayu¹, Dzul Haerah¹, Dan Wahyuni¹

This study aims to detect the presence of Staphylococcus aureus (S. aureus) and Streptococcus agalactiae (S. agalactiae) in subclinical mastitis infections in dairy cows and buffalos in Enrekang (in South Sulawesi, Indonesia). Subclinical mastitis was pre-examined using the California Mastitis Test (CMT) reagent, and 33 samples were detected as positive. The positive samples were then isolated with a culture test on the Baird Parker Agar media (BPA), identified with a catalyst, Gram staining, and a coagulase test, and isolated in Mannitol Salt Agar (MSA) media. To distinguish the S. aureus from others Staphylococcus species, the sample was then tested on blood agar media, in order to observe the presence of hemolysis. For the identification of S. agalactiae, culture methods and Gram staining were used, as well as biochemical tests using a catalyst, the Christie Atkins Munch-Peterson (CAMP) test, an eskulin bile test, glucose, maltose, sucrose, motility tests, an indole test, and finally urea and blood agar tests. The results showed that four samples were positive for S. aureus, characterized by grayish colonies observed on the BPA, while there were positive results in the gram staining (forming a bacteria chain of purple cocci), in the catalyst test, the coagulase test and the blood agar test. The glucose, maltose, and lactose tests were also positive, while the catalyst test, the eskulin bile test, the indole, and the urease test all showed negative results. The results also showed that S. agalactiae was detected in one milk sample, while the other 28 samples detected none of the two bacteria.

S. aureus, S. agalactiae, dairy cattle, dairy buffaloes