1General Commission for Scientific Agricultural Research, Hama, Syria
2Current address of Salah Eddin Khabbaz: A&L Biologicals, 2136 Jetstream Road, London, ON, N5V 3P5, Canada
3Department of Plant Pathology, Directorate of Rice Research, Hyderabad, India
4Department of Plant Pathology, Assiut University, Assiut, Egypt
World Journal of Agricultural Research.
2017,
Vol. 5 No. 2, 64-72
DOI: 10.12691/wjar-5-2-2
Copyright © 2017 Science and Education PublishingCite this paper: Salah Eddin Khabbaz, D. Ladhalakshmi, Ahmed Abdelmagid. Comparative Studies Between
Xanthomonas citri subsp
. malvacearum Isolates, Causal Agent of the Bacterial Blight Disease of
Cotton.
World Journal of Agricultural Research. 2017; 5(2):64-72. doi: 10.12691/wjar-5-2-2.
Correspondence to: Salah Eddin Khabbaz, General Commission for Scientific Agricultural Research, Hama, Syria. Email:
Salah_edk@yahoo.co.ukAbstract
Out of 21 bacterial isolates collected during a survey from seven different cotton growing areas representing three districts of Tamil Nadu, India, only eight bacterial isolates of Xanthomonas axonopodis pv. malvacearum (Xam), now being called (Xanthomonas citri subsp. malvacearum) were found to be pathogenic to the susceptible cotton variety TCB-209. The eight pathogenic isolates were grouped into clusters A and B based on protein profiles. Cluster A had I1, I3, I5, I7 and I8 isolates while cluster B had I2, I4 and I6 isolates.. The isolates were morphologically similar, but varied in their cultural and biochemical characteristics. Isolate I5 collected from Kavalur region showed a very high degree of virulence. Polyclonal antiserum was raised using the most virulent isolate I5 and the titre was found to be 1:500. The antiserum also reacted positively with the other isolates. Pathogen was detected from different parts of the infected plant through DAC-ELISA and the leaves recorded maximum absorbance value indicating high level of bacterial infection.
Keywords