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Zhang, W., Cheng, X. , Liu, Y. and Xiang, M., “Genome Studies on Nematophagous and Entomogenous Fungi in China,” J. Fungi, 2, 9 :1-14. Feb.2016.

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Article

Identification of Nematode Trapping Fungus Monacrosporium eudermatum Based on Genetic Diversity Using RAPD Technique

1Biological Department, College of Sciences, Misan University, Misan, Iraq


American Journal of Microbiological Research. 2016, Vol. 4 No. 6, 178-180
DOI: 10.12691/ajmr-4-6-4
Copyright © 2016 Science and Education Publishing

Cite this paper:
Ali A. Kasim, Maitham Dragh. Identification of Nematode Trapping Fungus Monacrosporium eudermatum Based on Genetic Diversity Using RAPD Technique. American Journal of Microbiological Research. 2016; 4(6):178-180. doi: 10.12691/ajmr-4-6-4.

Correspondence to: Ali  A. Kasim, Biological Department, College of Sciences, Misan University, Misan, Iraq. Email: alimycol@yahoo.com

Abstract

Nematode-trapping fungus Monacrosporium eudermatum a predacious fungus of nematodes, has been very useful in understanding the most relationship between nematophagous fungi and their nematode hosts. M.eudermatum is by far the common nematode-trapping fungus with the characteristic ability of forming adhesive trapping nets once in contact with nematodes. Total DNA was extracted from M.eudermatum sera and amplified by RAPD-PCR using three random primers (OPA-16, OPB-08, OPF-05). The results show amplification of all markers (OPF-05, OPB-08, OPA-16) with the genomic DNA studied. As evidenced by the results, all the markers covered in the study showed high polymorphism (up to 60%) with the exception of the primer (OPB-08) which showed no polymorphism.

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