1Biological Department, College of Sciences, Misan University, Misan, Iraq
American Journal of Microbiological Research.
2016,
Vol. 4 No. 6, 178-180
DOI: 10.12691/ajmr-4-6-4
Copyright © 2016 Science and Education PublishingCite this paper: Ali A. Kasim, Maitham Dragh. Identification of Nematode Trapping Fungus
Monacrosporium eudermatum Based on Genetic Diversity Using RAPD Technique.
American Journal of Microbiological Research. 2016; 4(6):178-180. doi: 10.12691/ajmr-4-6-4.
Correspondence to: Ali A. Kasim, Biological Department, College of Sciences, Misan University, Misan, Iraq. Email:
alimycol@yahoo.comAbstract
Nematode-trapping fungus Monacrosporium eudermatum a predacious fungus of nematodes, has been very useful in understanding the most relationship between nematophagous fungi and their nematode hosts. M.eudermatum is by far the common nematode-trapping fungus with the characteristic ability of forming adhesive trapping nets once in contact with nematodes. Total DNA was extracted from M.eudermatum sera and amplified by RAPD-PCR using three random primers (OPA-16, OPB-08, OPF-05). The results show amplification of all markers (OPF-05, OPB-08, OPA-16) with the genomic DNA studied. As evidenced by the results, all the markers covered in the study showed high polymorphism (up to 60%) with the exception of the primer (OPB-08) which showed no polymorphism.
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