1Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt
2Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt
3Department of Microbiology and Immunology, Faculty of Pharmacy and Drug manufacturing, Pharos University, Alexandria, Egypt
American Journal of Infectious Diseases and Microbiology.
2016,
Vol. 4 No. 1, 6-13
DOI: 10.12691/ajidm-4-1-2
Copyright © 2016 Science and Education PublishingCite this paper: Ghada F. Helaly, Sherine Shawky, Rania Amer, OlaAbdel- kader, Gamal El-Sawaf, Mohammed A. El Kholy. Expression of AcrAB Efflux Pump and Role of Mefloquine as Efflux Pump Inhibitor in MDR
E.coli.
American Journal of Infectious Diseases and Microbiology. 2016; 4(1):6-13. doi: 10.12691/ajidm-4-1-2.
Correspondence to: Sherine Shawky, Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt. Email:
sherineshawky@hotmail.comAbstract
Multi-drug resistant Escherichia coli (MDRE. coli), represents a major health problem. The AcrAB pump constitutes a major drug efflux system. Inhibition of efflux pumps is an approach to combat the drug resistance problem. Mefloquine is a bacterial RND efflux-pump inhibitor (EPI). This study aimed to investigate the expression levels of multidrug efflux genes acrAB, as well, to study the effect of mefloquine as an efflux pump inhibitor. acrA and acrB gene expression was measured using real-time PCR. Effect of mefloquine hydrochloride on minimum inhibitory concentration (MIC) of selected antibiotics against the tested isolates was determined using the broth microdilution technique. Overexpression of both genes was detected in all isolates. The differences between expression levels of both genes in MDR strains and an ATCC reference strain were statistically significant (p ˂ 0.001). There was a moderate correlation between acrA and acrB genes expression levels. (r = 0.593, p < 0.001). The isolates showed a decrease in MIC in presence of EPI. Susceptibility to levofloxacin was recovered in 95% of the tested isolates, 62.5% to ceftriaxone and in 5% each to ciprofloxacin and Gentamicin. In conclusion, Inhibition of acrAB efflux pump in MDR E. coli by mefloquine could be considered in the design of future antibiotics.
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