Mustafa Duzyol^1,, Murat Karamese², Nilgun Akgul³, Pinar Gul³, Neslihan Celik³, Esra Duzyol⁴, Ulku Altoparlak⁵

Objective: In this study, we compared the antibacterial effects of several dentin bonding agents with different pH values and active monomers. Method and Materials: Infected dentin samples were obtained from depths of approximately 4-6 mm, from isolated and air-dried dental caries. Streptococcus mitis was isolated and incubated on sheep blood agar plates at 37°C for 18 h. Impregnated antimicrobial disks were used for the liquids, and the samples were grouped as follows: group 1 was a negative control group with nothing applied; group 2 was a positive control group with 2% chlorhexidine digluconate applied; group 3 had Adper Single Bond Universal applied; group 4 had Clearfil SE Bond 2 (including 10-methacryloyloxydodecyl dihydrogen phosphate[MDP]) applied; group 5 had Clearfil S3 Bond Plus (including [MDP])applied; and group 6 had Clearfil Protect Bond (including 12-methacryloyloxydodecylpyridinium bromide [MDPB])applied. The antimicrobial disks were inserted into the blood agar plates. Inhibition zones were measured on the plates by well-educated specialists. Results: The 2% chlorhexidine digluconate solution had a more extensive inhibition zone than the other groups. S. mitis was significantly inhibited on the MDPB-impregnated disks applied to the agar plates. Conclusions: The results demonstrated that dentin bonding agents including MDPB-containing primer had significant antibacterial effects.

dentin bonding agents, MDPB, antimicrobial effect, agar-disk diffusion, tryptophan-containing broth