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Wilson, K. and Goulding, K. (1986): Principles and Techniques of practical biotechnology. London: Edward Amold Ltd.

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Production and Estimation of Keratinase by Immobilized and Free Bacillus licheniformis (St. 24)

1Microbiology Laboratory, Department of Botany, Omar Almukhtar University, El-beida, Libya

2Department of Botany, Faculty of Science, Minia University, Minia, Egypt

3Biology Department, Faculty of Science, Taif University, Taif KSA

4Department of Botany, Faculty of Science, Assiut University, Assiut , Egypt


Journal of Applied & Environmental Microbiology. 2015, Vol. 3 No. 5, 119-122
DOI: 10.12691/jaem-3-5-2
Copyright © 2016 Science and Education Publishing

Cite this paper:
Farag M. Saieb, Salama A. Boaker, Hasham M. El-komy, Ahmed Issa. Production and Estimation of Keratinase by Immobilized and Free Bacillus licheniformis (St. 24). Journal of Applied & Environmental Microbiology. 2015; 3(5):119-122. doi: 10.12691/jaem-3-5-2.

Correspondence to: Ahmed  Issa, Biology Department, Faculty of Science, Taif University, Taif KSA. Email: aaissaeg@gmail.com, aaissaeg@yahoo.com

Abstract

Alginate immobilized Bacillus licheniformis (St. 24) were isolated from chicken feather wastes recorded higher keratinase production than free bacterial suspension. 2-3% alginate concentration, medium pellet size, and in addition of keratin powder as adjuvant were optimum for keratinase production by the immobilized bacteria. Encapsulated B. licheniformis st. 24 was successfully produced keratinase for three repeated batch fermentation cycles each 24 hour incubation period. Immobilization of the whole cells proved to be useful for continuous production of keratinase and feather degradation.

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