Zainuddin AS^1,, Massi MN², Alam G³, Djawad K⁴, Yusuf I²

Aims. TheUVB light radiation inducts 8-OHdG as the photoproduct in which resulting the DNA impairment through stress oxidative pathway. Genistein is one of the basic component from soybean that the isoflavone inside conducts strong antioxidant effect. This study main purpose is to see the application of genistein’s photoprotective instrument on UVB induced-hairless mice. Method. 24 albino mice, age 6-9 weeks, weight 15-25 grams were used as the sample of the study. These mice were divided into 6 different way of treating groups, group I as the control group which had been selected as the negative control—untreated ones, group II as the control group which had the UVB radiation, group III as UVB radiation control group with Aceton-based, group IV which had 100 ppm genistein. Group V had 200 ppm genistein and group VI had UVB radiation 3 times a week with 500 mJ dosage for 12 weeks. After 24 hours of the last UV light exposure giving, these mice were killed off to aim the skin biopsy for 8-OHdG laboratory examination. Result. 8-OhdG level at 100 ppm genistein control group was the lowest one rather than the UVB radiation and 400 ppm control group. And, it’s not significantly showed at 200 ppm genistein control group. Conclusion. 100 ppm, 200 ppm and 400 ppm of genistein application showed the protective effect to 8-OHdG on UVB induced-hairless mice, which the best dose usage was 100 ppm of genistein.

Genistein, 8OHdG, Ultraviolet B