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Article

Molecular Diversity Assessment in Chickpea through RAPD and ISSR Markers

1School of Studies in Biotechnology, Jiwaji University, Gwalior, India

2ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India

32ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India


World Journal of Agricultural Research. 2015, Vol. 3 No. 6, 192-197
DOI: 10.12691/wjar-3-6-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Sameer. S. Bhagyawant, Neha Gupta, Ajay Gautam, S.K. Chaturvedi, Nidhi Shrivastava. Molecular Diversity Assessment in Chickpea through RAPD and ISSR Markers. World Journal of Agricultural Research. 2015; 3(6):192-197. doi: 10.12691/wjar-3-6-2.

Correspondence to: Nidhi  Shrivastava, 2ICAR-Indian Institute of Pulses Research, Kanpur, India 3Department of Bioscience & Biotechnology, Banasthali University, India. Email: nidhishrivastava@gmail.com

Abstract

Chickpea (Cicer arietinum L.) is a cool season food legume in the family of Fabaceae. The exploration of genetically variable accessions is the key source of germplasm conservation for future selecting parents for breeding. PCR based analysis employing RAPD and ISSR marker are one of the established techniques of estimation of genetic diversity. Twelve chickpea accessions were analyzed by ten RAPD and ISSR primers of which all primers gave amplification products. The results showed that the level of DNA polymorphism in these accessions is low. RAPD analysis yielded 5.9 bands per primer while ISSR analysis produce 2.8 bands per primer. The average percentage of molecular polymorphism as produced by RAPD and ISSR primers was 67%. The cluster dendrogram demonstrated a similarity coefficient range of 0.10 to 0.66 due to RAPD markers, whereas ISSR markers showed 0.60 to 0.80. The variability index worked out in the present study ranges from 0.49 to 0.78. The present study determines DNA polymorphism in chickpea accessions as revealed by RAPD and ISSR markers. Overall the study ascertained that RAPD and ISSR marker provide powerful tools in revealing genetic diversity in chickpea.

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