Sesilia Rante Pakadang1,
Chatarina Umbul Wahjuni2,
Hari Basuki Notobroto2,
Dwi Winarni3,
Ressy Dwiyanti4, 5,
Yadi6, 5,
Muhammad Sabir4, 5,
Mochammad Hatta5,
1Health Technology Department, Ministry of Health, Makasssar, Indonesia
2Faculty of Public Health, Airlangga University, Surabaya, Indonesia
3Faculty of Life Sciences and Technology, Airlangga University, Surabaya, Indonesia
4Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
5Molecular Biology and Immunology Laboratory for Infectious Diseases, Microbiology Department, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
6Department of Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
American Journal of Microbiological Research.
2015,
Vol. 3 No. 4, 129-134
DOI: 10.12691/ajmr-3-4-2
Copyright © 2015 Science and Education PublishingCite this paper: Sesilia Rante Pakadang, Chatarina Umbul Wahjuni, Hari Basuki Notobroto, Dwi Winarni, Ressy Dwiyanti, Yadi, Muhammad Sabir, Mochammad Hatta. Immunomodulator Potential of Miana Leaves(
Coleus scutellarioides (L) Benth)
in Prevention of Tuberculosis Infection.
American Journal of Microbiological Research. 2015; 3(4):129-134. doi: 10.12691/ajmr-3-4-2.
Correspondence to: Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infectious Diseases, Microbiology Department, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia. Email:
hattaram@indosat.net.idAbstract
Aim, The aim of this study to investigate the immunonomodulator effects of miana leaves (Coleus scutellarioides (L) Benth) on prevention of tuberculosis in wistar rats, Method, Samples of white male Wistar rats were divided into 4 groups, the samples were treated with miana leaves extract (EDM) and then infected by intra tracheal M. tuberculosis H37Rv strain and subsequently given a placebo, EDM and GAB (combined Rifampicin and EDM) and healthy control animals were treated with EDM. In this study we measure the level of Immunomodulatory parameter; the number of T-lymphocytes and CD4 T-cells measured by flowcytometry method, the levels of IFN-γ and TNF-α were measured by ELISA and the number of M. tuberculosis colonies derived from the rat lung in LJ media. Result, Results showed the increasing of T-lymphocytes, CD4 T-Cells, the levels of IFN-γ, TNF-α and decreasing in the number of M. tuberculosis colonies after EDM treatment. Conclusion, Miana leaves extract (Coleus scutellarioides, (L) Benth.) increased the number of T-lymphocytes, CD4 T-cell counts, levels of IFN-γ and TNF-α and decreased in the number of M. tuberculosis colony in infected wistar lung.
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