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Molecular Study of Panton-Valentine Leukocidin Genes among Staphylococcus aureus Clinical Isolates in Khartoum State, Sudan

1Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan

2College of Applied Medical Science, Shaqra University, KSA

3Department of Microbiology, Tropical Medicine Research Institute, National Center for Research, Sudan

4Department of Microbiology, Faculty of Medicine, King Khalid University, KSA

5Department of Biotechnology, Biotechnology Park, Africa City of Technology, Sudan


American Journal of Microbiological Research. 2015, Vol. 3 No. 3, 107-111
DOI: 10.12691/ajmr-3-3-2
Copyright © 2015 Science and Education Publishing

Cite this paper:
Najem Aldin M. Osman, Intisar E. Alrayah, Yassir Mahgoub Mohamed, Ali M El-Eragi, Muataz M. Eldirdery, Mohamed Ahmed Salih. Molecular Study of Panton-Valentine Leukocidin Genes among Staphylococcus aureus Clinical Isolates in Khartoum State, Sudan. American Journal of Microbiological Research. 2015; 3(3):107-111. doi: 10.12691/ajmr-3-3-2.

Correspondence to: Najem  Aldin M. Osman, Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan. Email: najemosman@hotmail.com

Abstract

Staphylococcus aureus strains carrying Panton- Valentine Leukocidin genes (PVL) are an emerging threat worldwide, causing variety of infections even in healthy individuals. Intensive efforts through the last years have been carried out towards the detection and analysis of PVL genes. The prevalence and characterization of such genes has not been done in Sudan. In this study we investigated the prevalence and the molecular characteristics of PVL genes among S. aureus clinical isolates, comparing their PVL allelic variant with that of PVL positive strains from different countries. Standard microbiological procedures were used for the identification of isolates, polymerase chain reaction for determination of PVL genes and standard sequencing for mapping of lukS/F-PV genes. In Silico tools were used for sequence analysis. Among 210 S. aureus isolates, PVL genes were detected in 122 (58%). Sequence analysis for lukS/F-PV genes from 12 representative isolates detected a new point mutation in lukS PV region. Collectively, our findings showed a high frequency of PVL genes among S. aureus isolates and revealed a novel nonsynonymous mutation. Phylogenetic analysis revealed that Sudanese isolates were closely related to each other.

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