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Orend, G. 2005a, “Potential Oncogenic Action Of Tenascin-C In Tumorigenesis”, International Journal Of Biochemistry & Cell Biology, Vol. 37, No. 5, Pp. 1066-1083.

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Article

Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression

1Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia

2Department of Medical Biochemistry, National Research Center, Cairo, Egypt


American Journal of Medical and Biological Research. 2015, Vol. 3 No. 2, 62-67
DOI: 10.12691/ajmbr-3-2-3
Copyright © 2015 Science and Education Publishing

Cite this paper:
Ali S. Alharth, Sherien M. El-Daly. Effects of Tenascin-C (TNC) Knockdown on Global Genes Expression. American Journal of Medical and Biological Research. 2015; 3(2):62-67. doi: 10.12691/ajmbr-3-2-3.

Correspondence to: Ali  S. Alharth, Department of Public Health, Ministry of Health, Riyadh, Saudi Arabia. Email: alharth_a@yahoo.com

Abstract

The extracellular matrix protein tenascin-C (TNC) is up-regulated in many cancers including breast cancer. TNC is associated with tumour progression and poor prognosis. The aim of this study was to investigate the effects of TNC knockdown on global gene expression in TNC expressing invasive breast cancer cell lines. Breast cancer cell lines (MDA-MB-231 and MDA-MB-436) were transfected with small interfering RNA (siRNAs) targeting total TNC. cDNA microarray was used to analyse the effects of TNC knockdown on global gene expression at the mRNA level. Microarray analysis following total TNC knockdown revealed significant changes in gene expression: CREBL2, YWHAE and RRAS2 showed down regulation and QKI was specifically up-regulated. In conclusion, the silencing of TNC expression caused significant alteration on global genes expression associated with tumour progression.

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