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Spectral Determination of Components Isolated from the Root of Ximenia Americana Linn

OUMAR A. ADOUM

Department of Pure and Industrial Chemistry, Bayero University, P.M.B. 3011, Kano, Nigeria

Abstract: One steroid, β-sitosterol (I), and two carboxylic fatty acids stearic (2), and trans-4-octadecenoic (3) acids were isolated from the roots of Ximenia americana Linn. (Olacaceae). Trans-l4-octadecenoic acid exhibited in-vitro cytotoxicity against brine shrimp larvae of Artemia salina (LC₅₀ = 79.µg/ml). Compounds 1 and 2 showed no activity in brine shrimp lethality test (LC₅₀> 1000µg/ml). ¹H and ¹³C NMR, IR and MS, spectroscopy have been used jointly to determine the conformation of the three compounds.

Keywords: Ximenia americana, β-sitosterol, fatty acids, Artemia salina, brine shrimp

Citation: OUMAR A. ADOUM, Spectral Determination of Components Isolated from the Root of Ximenia Americana Linn. World Journal of Organic Chemistry, 3 (1). 12-15. January 2015. doi:10.12691/wjoc-3-1-3

1. Introduction

Ximenia americana 'Tsada" (Hausa) and "Chabulli" (Fulfulde) is a shrub with white flowers which grows mainly in the African savanna [4,7,8].

The root of the plant is used to treat leprosy, dysentery, and mental sickness. The Hausawa and Fulani of northern Nigeria use the roots of Ximenia americana and that of Annona senegalensis to treat trypanosomiasis, and also eye and ear infections. In Angola the crushed bark is applied to sores of domestic animals [13].

Sambunigirin was isolated from the leaves of Ximenia americana [3]. The following acids have also been detected in the seed oil of Ximenia plant: Oleic (60.8%). cerotic (15.21%), ximenic (14.6%), linoleic (6.7%), and stearic acid (1.2%) [11]. Trans-13-octadecen-9, 11-dynoic acid, trans-l l-octadecen-9-ynoic acid (Ximeninic acid), trans-l 1-trans- 13-octa decadien-9-ynoic acid and oleanolic acid saponin have been isolated from the roots [1,5].

Early pharmacological report on the plant have concluded that the roots have no antimalarial activity and the extracts of the fruits, twigs and leaves showed no significant insecticidal property [6,14].

Herein we report on the bioactivity-guided isolation and the identification of two fatty acids (2) and (3) in the roots of Ximenia americana. The identity of β-sitosterol (1) was confirmed' by comparison of its spectral data with those reported in the literature.

2. Experimental Procedure

Apparatus and Reagents: Solvents were used without further purification except some ethanol which was redistilled before use. Column chromatography was performed on silica gel (Merck 70 - 30 mesh, bet surface area 500m²/g, pore volume 0.75cm²). Thin layer chromatography (TLC) was performed on plates coated with silica gel (Merck, TLC grade, with gypsum binder and fluorescent indicator). TLC bands were visualized under UV light (at 254 nm and 365 nm) or by exposure to iodine. Brine shrimp eggs (Artemia. Inc., California) and instant ocean see salt were purchased from Aquarium systems, Ohio, USA.

EIMS were obtained on JEOL IMS - 5 x 102 A spectrometer. ¹H and I3C NMR were recorded on JEOL JNM - EX 400 spectrophotometer and Bruker Avance in CDL₃, TMS was used as standard in NMR measurements. IR was visible HP-8453 spectrophotometer.

Plant Material. The root of X. americana was collected at Yako Village, 40 KM from Kano. northern Nigeria. The material was authenticated by Baba Ali Garko of the Herbarium of Bayero University, Kano. The root was dried and crushed into a fine powder.

Brine Shrimp Lethality (BST). Extracts, fractions and isolated compounds were evaluated for lethality to brine shrimp larvae [9,10]. In this test, a drop of DMSO was added to test and control vials to enhance the solubility of test materials.

Extraction and Isolation. Dried and ground root of X. americana (3.355kg) was percolated with 95% ethanol/chloroform (1:1) for two weeks, after the extract was drained off and evaporated in vacuum at 40°C. The marc was percolated with 6.5 litres of 95% ethanol/chloroform (1:1) for another one week and then drained and evaporated. The combined residue, 86.49g, reddish-brown labeled FOO1 was stored in a freezer until used. The column fractions of the crude extract (FOO1) of the root of X. americana was carried out by methods described by Fatope et al., [2], In this method three portions (25g each) of the crude extract (FOO1) were separately chromatographed on silica gel columns using different solvent mixtures. Fractions were pooled and screened against brine shrimp larvae. Identical eluents were combined based on their TLC patterns and activity in BST.

Activity guided fractionation led to the isolation of fraction XA-9-4 (1.1588g) which was also inactive in BST (LC₅₀ > 1000µg/ml). The fraction, a colourless oily substance was loaded on smaller column (length = 25cm, id = 2cm) packed with 30.5g silica gel. The column was eluted in the following order and collecting fractions in portions of 50ml: (50ml) petroleum ether, (1:1050ml) petroleum ether and chloroform. (1:15, 50ml) petroleum ether and chloroform (50ml) chloroform. (1:1, 50ml) chloroform and ethylacetate, and (50ml) ethyl acetate.

Eluents were pooled into nine fractions. Fractions XA-9-4-2. XA-9-4-3, XA-9-4-5, XA-9-4-7, XA-9-4-8 and XA-9-4-9 were pale yellow and XA-9-4-4 was a colorless oily fraction. TLC analysis revealed that XA-9-4-2 and XA-9-4-3 are the same compound at different levels of purity. Spectral data revealed that the dominant product in the two fractions is oleanene hydrocarbon and ester.

Further purification of (XA-9-4-4) gave colorless oil fractions. One of the fractions is coded XA-9-4-4-1. 0.1300g of fraction XA-9-4-4-1, a colourless semi solid, was loaded on a preparative TLC and developed in petroleum ether: ethyl acetate (15:1). Three major bands were obtained. The top XA-9-4-4-4-1A [35mg, Rf 0.36 in petroleum ether: ethyl acetate (15:1)] and the middle XA-9-4-4-IB (41 mg. R, 0.24 in petroleum ether: ethyl acetate (15:1)] were single products: and the bottom (XA-9-4-4-1C) a mixture of several compounds. Fractions XA-9-4-4-1A and XA-4-4-1B are inactive in BST (LC₅₀ > 1000µg/ml).

Six fractions obtained from XA-9-5 (0.9509g) were pooled and purified on preparative TLC, eluting with chloroform: ethyl acetate (1:1), to give 2 (XA-9-5-5B). Fraction XA-9-5-6 (0.1232g) was loaded on preparative TLC and developed in chloroform: ethyl acetate (1:1). When the plate was visualized under UV light at 254 nm. two major bands were observed and the top band was compound 3 (XA-9-5-6A) scraped off, dissolved in ethyl acetate, filtered and dried. 3 (44mg) was a yellow oil.

β-sitosterol (1) (XA-9-4-4-1 A) was obtained as a white semi solid (35mg);EI-MS/mz (relative intensity) 414 (78) [M⁺], 396 (70) [M-H₂O]⁺, 381(30) [M-CH₃]⁺. 329 (30). 107 (49), 105 (40), 95 (59). 93 (36), 91 (33). 81 (61), 67 (36); HR El-MS m/z 414. 3854 (for C₂₉ H₅₀ O);¹ H NMR (CDCL₃ 400 MHz) gave signals at δ1,007 (3H. d. J =6.8 Hz. 4H-21), 0.845 (3H. t. J =7.2 Hz, 3H, 29, 0.836 (3H, J = 6.8 Hz 3H - 26), 0.815 (3H, d, J = 6.8 Hz. 3H - 27); ¹³C NMR (CDC1₃, 100MHz) data see Table 1. The ¹H NMR spectral data were consistent with reported values [12].

Stearic Acid (2) was obtained as a yellow oil (4lmg); IR (film) (cm-¹) 2926. 2857. 2230, 1714, 1465, 1242, El-MS m/z (relative intensity) [M⁺] 284 (51), 256 (100) [M-CO]⁺, 99 (100) [M-CH₂(CH₂)₁₀CO₂H]⁺, 71 (100) [M-CH₂ (CH₂)₁₁CO,H]⁺, 57(30) [M-CH₂(CH₂)₁₂CO₂H]⁺, 43 (100) [M-CH₂(CH₂)₁₃CO₂H)⁺; HR El-MS m/z 284.27l9[M⁺] (calcd 284.2715 for C₁₈H₃₆O₂); ¹HNMR(CDC1₃.400MHz.) data see Table 2 and Figure 1.

Figure 1

14-octadecenoic Acid (3) was obtained as a yellow oil (44mg), IR (film) (Cm⁻¹) 2935, 2820, 1723, 1474, 1294, 950, 718, El-MS m/z (relative intensity) [M⁺] 282 (25), 264 (63) [M-H₂0]⁺, 11(76) [M-CH₂(CH₂)₈CO₂H]⁺ 83 (93) [M-CH₂(CH₂)₁₀CO₂H]⁺, 69 (80) [M-CH₂(CH₂)₁₁CO₂H⁺]⁺, 43(100) [M-CH₂ (CH₂)₁₃ CO₂H]⁺, HR El-MS m/z 282.2554 [M⁺] (calcd 282.2514 for C₁₈H₃₄O₂). ¹H NMR (CDI₃, 400 MHz) and ¹³CNMR (CDC1₃, 100MHz) data see Table 2 and Figure 1.

Table 1. ¹³CNMR (100 MHz, CDl₃) data for compound XA-9-4-4-1A

Table 2. ¹H (400 MHz. CDCl₃ and ¹³C (100MHz. CDCI₃) NMR Data for 2 and 3

3. Results and Discussion

β-sitosterol (1) in had a molecular formula of C₂₉ H₅₀ O. The molecular ion was indicated by a dominant peak at m/z 414 [M⁺] in El-MS. The peaks at m/z (relative intensity) 396 (70) and 381 (30) correspond to the loss of a water molecular ion. The El-MS also showed some characteristics peaks at m/z 107 (49), 105 (95), 93 (36), 91 (33), 81 (61) and 67 (36). The HR El-MS gave m/z 414.3854 and unsaturarion number of 5.0 for [M⁺].

The ¹H NMR spectrum displayed diagnostic signals due to the influence of the ring system on the side chain methyl groups in the compound: 3H singlets at δ 1.007 and 0.68 (each 3H, S, CH₃ x 2) assigned to 3H - 26: 3H doublet at δ 0.815 (3H, J = 6.8 Hz) assigned to 3H - 27. The summary of ¹³C NMR assignment is presented as in Table 1.

Stearic acid (2) had a molecular formula of C18 H34 O₂. The molecular ion was indicated by the peak at m/z 284 [M+] in El-MS. It also showed some peaks at m/z 99 (100). 85 (100), 85 (100), 71 (100), 57 (30), and 43 (100) corresponding to the loss of 185, 199, 213, 227 and 241 mass units from the molecular ion [M+]. The IR spectrum displayed an acid band at 1714cm⁻¹. The ¹H NMR showed the presence of the following easily recognized peaks: 2H triplet at δ 2.34 (2H, t, J = 7.2Hz) assigned to H-2: triplet at δ. 0.88 (3H. t, J = 6.8Hz) assigned to H-I8:2H multiple at δ 1.63-1.31 (2H, m) assigned to 15 equivalent methylene hydrogens.

The summary of ¹³C NMR (see Figure-I) assigned is presented as in Table 2.

Trans-14-octadecenoic acid (3) had a molecular formular of C₁₈ H₃₄ O₂. The molecular ion was indicated by the peak at m/z 282 (M+) in El-MS. The HR El-MS gave rn/z 282.2554 (calcd 282.2514) for [M+] and showed unsaturation number of 2.0. The IR displayed an acid band at 1723cm and unsaturation band (alkene C-H def) at 950cm⁻¹.

The presence of the double between C-14 and C-15 was supported by ¹³C NMR signals at δ 2.06 (2H, m), 2.13 (2H. m) and 5.34 (IH. m). The placement of the double bond between C-14 and C-15 was further supported by the mass spectra peaks at m/z 43 (100), 69 (80), and 97 (100), corresponding to the loss of 239, 213 and 185 mass units from the molecular ion [M⁺]. The summary of ¹³C NMR spectral data as in Table 2.

Acknowledgement

The author would like to thank Prof. M.O. Fatope, Chemistry Department. Sultan Qaboos University, Sultanate of Oman and Prof. Yoshio Takeda, Faculty of Integrated Arts and Sciences. The University of Tokushima, Tokushima, Japan for the tremendous assistance rendered to accomplish this work.

References

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