Science and Education Publishing World Journal of Chemical Education 2 4 2014 12 25 The Identification of Amino Acids by Interpretation of Titration Curves: An Undergraduate Experiment for Biochemistry 59 61 EN Cassidy M. Dobson Nathan S. Winter Department of Chemistry and Biochemistry, St. Cloud State University, St. Cloud, Minnesota, United States WJCE2014243 10.12691/wjce-2-4-3 2014 11 08 2014 12 08 2014 12 25 Undergraduate biochemistry students should have great familiarity with titration curves. These curves allow the prediction of protonation states, charges, and isoelectric points. Here we describe an experiment in which students identify four amino acids based on their titration behavior. Students make solutions of each unknown amino acid and monitor the change in pH upon adding aliquots of a strong base. They identify the amino acids based on the shapes of the curves. They annotate the plots with isoelectric points, pKas, buffering regions and the structures of the amino acids.