@article{wjar2017522,
author={{Khabbaz, Salah Eddin and Ladhalakshmi, D. and Abdelmagid, Ahmed},
title={Comparative Studies Between <i>Xanthomonas</i> <i>citri</i> subsp<i>.</i> <i>malvacearum</i> Isolates, Causal Agent of the Bacterial Blight Disease of<i> </i>Cotton},
journal={World Journal of Agricultural Research},
volume={5},
number={2},
pages={64--72},
year={2017},
url={http://pubs.sciepub.com/wjar/5/2/2},
issn={2333-0678},
abstract={Out of 21 bacterial isolates collected during a survey from seven different cotton growing areas representing three districts of Tamil Nadu, India, only eight bacterial isolates of <i>Xanthomonas axonopodis </i>pv. <i>malvacearum </i>(<i>Xam</i>), now being called (<i>Xanthomonas citri </i>subsp<i>. malvacearum</i>) were found to be pathogenic to the susceptible cotton variety TCB-209. The eight pathogenic isolates were grouped into clusters A and B based on protein profiles. Cluster A had I<SUB>1</SUB>, I<SUB>3</SUB>, I<SUB>5</SUB>, I<SUB>7</SUB> and I<SUB>8</SUB> isolates while cluster B had I<SUB>2</SUB>, I<SUB>4</SUB> and I<SUB>6</SUB> isolates.<SUB>.</SUB> The isolates were morphologically similar, but varied in their cultural and biochemical characteristics. Isolate I<SUB>5</SUB> collected from Kavalur region showed a very high degree of virulence. Polyclonal antiserum was raised using the most virulent isolate I<SUB>5</SUB> and the titre was found to be 1:500. The antiserum also reacted positively with the other isolates. Pathogen was detected from different parts of the infected plant through DAC-ELISA and the leaves recorded maximum absorbance value indicating high level of bacterial infection.},
doi={10.12691/wjar-5-2-2}
publisher={Science and Education Publishing}
}