@article{wjar2014223,
author={{Abdalla, Saifeldeen A. and Algam, Soad A. A. and Ibrahim, Elshiekh A. and Naim, Ahmed M. El},
title={In Vitro Screening of Bacillus Isolates for Biological Control of Early Blight Disease of Tomato in Shambat Soil},
journal={World Journal of Agricultural Research},
volume={2},
number={2},
pages={47--50},
year={2014},
url={http://pubs.sciepub.com/wjar/2/2/3},
issn={2333-0678},
abstract={Rhizosphere bacteria are one of the most potential biological control agents in the plant disease protection. <i>Bacillus </i>species as a group offer several advantages over other bacteria for protection against pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. Five soil samples from tomato rhizosphere were collected from shambat area, Khartoum State, Sudan. <i>Bacillus</i> isolates were isolated from the rhizosphere of tomato to use as natural bio-control agents. They were screened for antagonism <i>in vitro</i> against <i>Alternaria alternata</i> causal agents of early blight disease of tomato. Serial dilution technique was adopted for the isolation of <i>Bacillus</i> species. Only 27 out of 45<i> Bacillus</i> isolates showed antagonistic properties. Four out of the 27 isolates showed antagonism (Bacillus B25, B35, B41, B45) were identified to the species level by bacteriological assay (morphological and biochemical tests).},
doi={10.12691/wjar-2-2-3}
publisher={Science and Education Publishing}
}