eng Science and Education Publishing World Journal of Agricultural Research 2333-0678 2023-03-26 11 1 30 38 10.12691/wjar-11-1-5 WJAR20231115 article Kamal Hassan Suliman 1 Fahad Nasser Al-Barakah 2 Abdulaziz Muhmmad Assaeed 2 Elgodah H. Ahmed 2 Seif Aldin Dawina Abdallah Fragallah 2 Elshiekh A.Ibrahim 2 Ahmed M. El Naim naim17amn@yahoo.com 2 Faculty of Natural Resources and Environmental Studies, University of Kordofan. P.O Box 160, El Obied, Sudan College of Food and Agriculture Sciences, King Saud University, P.O. Box; 2640, Riyadh, Saudi Arabia To assess mycorrhizal fungi efficiency on Acacia growth performance under water shortage condition, three leguminous plant species (Acacia tortilis, Acacia ehrenbergiana and Acacia gerrardii) were selected under greenhouse conditions in washed soil. The mycorrhizal fungal colonization was used to enhance plants growth under water deficit. Three watering levels; 85%, 75%, 50% and 25% of Field Capacity (FC) in the presence of Mycorrhizal and non-Mycorrhizal applied on grown trees for 5 months. This treatment impact on the plants was assessed by comparing plants heights, number of leaves shoot, root fresh, dry weight and Relative Growth Rate (RGR), and by measuring mycorrhizal colonization percentage and intensities. The results indicated that Arbuscular Mycorrhizal Fungi (AMF) significantly increased colonization percentage irrespective of acacia species. The maximum of root colonization percentage obtained at 75 % FC. Greater mycelium infection was observed at A. tortilis, A. gerrardii and A. ehrenbergiana (88.1%, 87.4%, and 86.4% respectively) at FC 75%, while the mycelium infection decreased at FC 25% at all species. The maximum vesicles were found with A. ehrenbergiana, A. gerrardii and A. tortilis (85.3%, 73.2%, and 53.5% respectively) at 75% FC, while the highest infection of Arbuscular (33.6%) was recorded with A. ehrenbergiana under 75% FC. Colonization intensity % significantly affect A. gerrardii registered highest mycelium intensity (66.3%) amended with 75% FC. The greater vesicles infection (62.6%) recorded with A. ehrenbergiana at the same FC, while maximum Arbuscular density (35.7%) with A. ehrenbergiana under 75% FC. Irrespective of Acacia species mycorrhizal fungi significantly enhanced the trees growth (plant height, leaves, shoot and root fresh weight, shoot dry weight and RGR) at 75% FC. http://pubs.sciepub.com/wjar/11/1/5/wjar-11-1-5.pdf water stress acacia mycorrhizal growth inoculum