@article{wjar20221014,
author={{Banga, Alvychelle Benith and Lebonguy, Augustin Aim¨¦ and Boumba, Ang¨¦lique Esp¨¦rance Lembella and Goma-Tchimbakala, Joseph},
title={Bacterial Communitie¡¯s Diversity of Rhizosphere¡¯s Soils of Two Legumes, <i>Cajanus cajan</i> and <i>Milletia laurentii</i>, Revealed by Illumina Miseq Sequencing of 16S rRNA Gene},
journal={World Journal of Agricultural Research},
volume={10},
number={1},
pages={20--29},
year={2022},
url={http://pubs.sciepub.com/wjar/10/1/4},
issn={2333-0678},
abstract={Microbial organic fertilizers have been shown to boost plant productivity. These microorganisms of interest are more numerous in the soil around the roots or rhizosphere. Objective of this study was to assess bacterial communities¡¯ diversity of in the rhizosphere of two legumes, <i>Milletia laurentii</i> and <i>Cajanus cajan</i>, growing on the same soil. First of all, the levels Mg, N, Fe, C total, P, NH<SUB>4</SUB><SUP>+</SUP> and particle size were determined by spectrophotometry, Kjeldahl method, Olsen method, Walkey-Black method, Nessler reagent, DEB method and Robinson pipette method, respectively. Next, bacterial diversity was determined by Sequencing Illumina Miseq of 16S rRNA gene. Results showed that contents of carbon, total nitrogen, ammoniacal nitrogen, phosphorus, iron and magnesium were slightly elevated in <i>Milletia</i> rhizosphere compared to <i>Cajanus</i>. According to the USDA's textural triangle, both soils have a sandy loam soil texture. In terms of diversity, all OTUs (1434) were divided into 30 phyla, 50 classes, 158 families and 314 genera for the 2 soils. Proteobacteria (58.62% - 48.71%), Acidobacteria (27.29% - 9.46%), Firmicutes (8.26% - 7.21%) and Bacteroidetes (13.70% - 2.53%) were most dominant phyla in both rhizospheres (<i>Cajanus</i> - <i>Milletia</i>). The most dominant classes were Alphaproteobacteria (51.44% - 38.90%), Acidobacteriia (26.57% - 8.67%), Bacilli (8.19% - 7.18%), Sphingobacteria (9.83% - 2.50%) and Gammaproteobacteria (4.27% - 3.39%). At the family level, Hyphomicrobiaceae (35.05%-24.22%), Bradyrhizobiaceae (17.32%-11.70%) and Bacillaceae (18.98%-6.49%) were most abundant. Finally, <i>Acidobacterium</i> (26.55%-4.58%), <i>Rhodoplanes</i> (21.63%-7.50%), <i>Bradyrhizobium</i> (17.27%-1.96%) and <i>Bacillus</i> (6.43%-6.29%) were the most abundant genera. Thus, bacterial diversity of the rhizosphere of these two legumes encourages their use for the isolation of bacteria with biofertilizing potential.},
doi={10.12691/wjar-10-1-4}
publisher={Science and Education Publishing}
}