@article{wjar20221013,
author={{Waidyaratne, S.M. and Samanmalie, L.G.I. and Buddhinie, P.K.C.},
title={<i>In vitro </i>Propagation of <i>Carica papaya</i> L. Variety ¡®Horana Papaya Hybrid 01¡¯ Using Shoot Tip},
journal={World Journal of Agricultural Research},
volume={10},
number={1},
pages={15--19},
year={2022},
url={http://pubs.sciepub.com/wjar/10/1/3},
issn={2333-0678},
abstract={<i>In vitro</i> plantlet regeneration ability of the local hybrid papaya variety<i> </i>Horana Papaya Hybrid 01 was evaluated using shoot tips from greenhouse-raised seedlings. Shoots tips surface sterilized in 20% Sodium Hypochlorite (Clorox<SUP>?</SUP>) for 20 min were established in Murashige and Skoog (MS) basal medium containing 6-Benzylaminopurine (BAP; 0.0,0.5,1.0,1.5 mg/L) in combination with 1-Naphthalene Acetic Acid (NAA; 0.0,0.1,0.5 mg/L)<i> </i>for shoot multiplication. Considering the high mean number of shoots per explant (4.8 ¡À 0.5) and absence of calli, 1.0 mg/L <SUP> </SUP> BAP was the best treatment for direct organogenesis. Proliferated shoots were transferred to a 1.5 strength MS medium containing 0.25 mg/L BAP and Gibberellic Acid (GA<SUB>3</SUB>; 0.0, 0.15, 0.30 mg/L) for further elongation. Elongated shootlets were placed in half-strength MS medium supplemented with Indole-3-Butyric Acid (IBA; 0.0,1.0, 2.0, 3.0 and 4.0 mg/L) for rooting. The highest root induction response (86%) and roots suitable for acclimatization were observed with 2.0 mg/L of IBA. Plantlets were acclimatized in poly cups containing a sterile potting mixture (soil, sand, compost, and coir dust; 1:1:1:1) and a survival rate of 75% was achieved under <i>in vitro</i> conditions. The findings of the present study can be optimized to develop a suitable <i>in vitro</i> micropropagation protocol for rapid clonal propagation of this papaya variety for producing true to type planting material.},
doi={10.12691/wjar-10-1-3}
publisher={Science and Education Publishing}
}