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American Journal of Public Health Research
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The Implementation of a Tele-Homecare System with Service Oriented Architecture and HL7 Message Transmission Standard
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American Journal of Medical and Biological Research
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The Influence of Ethanol on Pyruvate Kinases Activity in Vivo, in Vitro, in Silico
Lelevich Sergey Vladimirovich, Khrustalev Vladislav Victorovich, Barkovsky Eugene Victorovich, Shedogubova Tatyana Aleksandrovna

American Journal of Medical Sciences and Medicine
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Clinical and Histological Features in Wegener Granulomatosis
Li Cavoli Gioacchino, Passantino Rita, Bono Luisa, Tortorici Calogera, Ferrantelli Angelo, Giammarresi Carlo, Rotolo Ugo

American Journal of Infectious Diseases and Microbiology
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The Curse of Lymphatic Filariasis: Would the Continual Use of Diethylcarbamazine Eliminate this Scourge in Papua New Guinea?
Anne Lanham, Lillian Mwanri

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Label_Title

Dear Label_Name,

Greetings! I hope you are well.

I am writing on behalf of an international publishing house, Science and Education Publishing, USA (http://www.sciepub.com).

Science and Education Publishing (SciEP) is a multidisciplinary open access publisher of Journals covering the fields of Science, Technology and Medicine. All SciEP publications are subject to high-quality peer review, editorial and production processes and are freely available online.

Welcome to the April, 2013 edition of SciEP newsletter, providing monthly summaries of articles on science & education related topics of interest to scientist and professionals. The mission of our journals is to provide an open access forum for publishing new findings on issues pertinent to scientific and academic field. We also invite you to submit original research papers, review articles, short reports, or case studies are considered for publication in our Multidisciplinary Journals.

American Journal of Public Health Research
DOI: 10.12691/ajphr-1-1-3

The Implementation of a Tele-Homecare System with Service Oriented Architecture and HL7 Message Transmission Standard
Ching-Sung Wang

American Journal of Medical and Biological Research
DOI: 10.12691/ajmbr-1-1-2

The Influence of Ethanol on Pyruvate Kinases Activity in Vivo, in Vitro, in Silico
Lelevich Sergey Vladimirovich, Khrustalev Vladislav Victorovich, Barkovsky Eugene Victorovich, Shedogubova Tatyana Aleksandrovna

American Journal of Medical Sciences and Medicine
DOI: 10.12691/ajmsm-1-1-1

Clinical and Histological Features in Wegener Granulomatosis
Li Cavoli Gioacchino, Passantino Rita, Bono Luisa, Tortorici Calogera, Ferrantelli Angelo, Giammarresi Carlo, Rotolo Ugo

American Journal of Infectious Diseases and Microbiology
DOI: 10.12691/ajidm-1-1-2

The Curse of Lymphatic Filariasis: Would the Continual Use of Diethylcarbamazine Eliminate this Scourge in Papua New Guinea?
Anne Lanham, Lillian Mwanri

We have released issues of some journals on February 2013.
American Journal of Public Health Research
American Journal of Medical Sciences and Medicine
American Journal of Medical and Biological Research
American Journal of Pharmacological Sciences
American Journal of Clinical Medicine Research
American Journal of Nursing Research
American Journal of Cancer Prevention
American Journal of Medicine Studies
American Journal of Infectious Diseases and Microbiology
American Journal of Cardiovascular Disease Research
American Journal of Biomedical Research
American Journal of Mechanical Engineering
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Journal of Preventive Medicine
American Journal of Food and Nutrition
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Dear Label_Name,

Greetings! I hope you are well.

I am writing on behalf of an international publishing house, Science and Education Publishing, USA (http://www.sciepub.com).

Science and Education Publishing (SciEP) is a multidisciplinary open access publisher of Journals covering the fields of Science, Technology and Medicine. All SciEP publications are subject to high-quality peer review, editorial and production processes and are freely available online.

Welcome to the April, 2013 edition of SciEP newsletter, providing monthly summaries of articles on science & education related topics of interest to scientist and professionals. The mission of our journals is to provide an open access forum for publishing new findings on issues pertinent to scientific and academic field. We also invite you to submit original research papers, review articles, short reports, or case studies are considered for publication in our Multidisciplinary Journals.

American Journal of Public Health Research
DOI: 10.12691/ajphr-1-1-3

The Implementation of a Tele-Homecare System with Service Oriented Architecture and HL7 Message Transmission Standard
Ching-Sung Wang

American Journal of Medical and Biological Research
DOI: 10.12691/ajmbr-1-1-2

The Influence of Ethanol on Pyruvate Kinases Activity in Vivo, in Vitro, in Silico
Lelevich Sergey Vladimirovich, Khrustalev Vladislav Victorovich, Barkovsky Eugene Victorovich, Shedogubova Tatyana Aleksandrovna

American Journal of Medical Sciences and Medicine
DOI: 10.12691/ajmsm-1-1-1

Clinical and Histological Features in Wegener Granulomatosis
Li Cavoli Gioacchino, Passantino Rita, Bono Luisa, Tortorici Calogera, Ferrantelli Angelo, Giammarresi Carlo, Rotolo Ugo

American Journal of Infectious Diseases and Microbiology
DOI: 10.12691/ajidm-1-1-2

The Curse of Lymphatic Filariasis: Would the Continual Use of Diethylcarbamazine Eliminate this Scourge in Papua New Guinea?
Anne Lanham, Lillian Mwanri

We have released issues of some journals on February 2013.
American Journal of Public Health Research
American Journal of Medical Sciences and Medicine
American Journal of Medical and Biological Research
American Journal of Pharmacological Sciences
American Journal of Clinical Medicine Research
American Journal of Nursing Research
American Journal of Cancer Prevention
American Journal of Medicine Studies
American Journal of Infectious Diseases and Microbiology
American Journal of Cardiovascular Disease Research
American Journal of Biomedical Research
American Journal of Mechanical Engineering
American Journal of Microbiological Research
Journal of Preventive Medicine
American Journal of Food and Nutrition
American Journal of Hypertension Research
American Journal of Sports Science and Medicine
Journal of Cancer Research and Treatment
American Journal of Zoological Research
Journal of Biomedical Engineering and Technology
American Journal of Microbiological Research
Biomedical Science and Engineering

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Biochemical Composition of Carapa procera seed Oil Harvested in Casamance, Southern Senegal

Djibril Diedhiou*, Mamadou Faye, Moussa Bagha Diedhiou, Adama Diop, Laure Candy, Virginie Buthod-Cuam

UFR Sciences Fondamentales et de l’Ingénieur, Université du Sine Saloum El Hadji Ibrahima Niass (USSEIN) - BP 55 Kaolack, Sénégal

Abstract: This study focuses on characterizing the biochemical composition of Carapa procera seeds oil harvested in Casamance. The kernel accounts for 71.23% of the seed, constituting the main fraction from which the oil is extracted. Analysis of the lipid profile reveals that the oil is dominated by oleic acid (45.7%), palmitic acid (32.75%), and linoleic acid (13.02%). This oil is composed of 97.22% triglycerides, distributed mainly among T52 (PLS) triglycerides (nearly 46%), T54 (LPO+LSLl) (about 30.5%), and T50 (OPPl+OSM) (nearly 20%), with minor fractions of T56 (OSA+OSG) (2.57%) and T48 (OPM) and T58 (OSB) (less than 1% each). The diglyceride content (C16 and C18) is 2.07%. The oil also has a phytosterol content of 9.09 g/kg, largely dominated by β-sitosterol (78%) and campesterol (16.17%), as well as a tocopherol content of 2.59 mg/kg, consisting mainly of γ-tocotrienol (nearly 49%), followed by α-tocotrienol (17.2%), α-tocopherol (15.73%), and δ-tocotrienol (12.72%). These results highlight an oil with a balanced lipid profile that is rich in minor bioactive compounds, particularly tocotrienols, reinforcing its potential value for cosmetic, nutraceutical, and pharmaceutical applications.

Keywords: Biochemical composition, Carapa procera, seeds, oil, Casamance, Senegal

Received August 05, 2026; Revised September 07, 2026; Accepted September 14, 2026

Citation: Djibril Diedhiou, Mamadou Faye, Moussa Bagha Diedhiou, Adama Diop, Laure Candy, Virginie Buthod-Cuam, Biochemical Composition of Carapa procera seed Oil Harvested in Casamance, Southern Senegal. World Journal of Analytical Chemistry, 11 (1). 20-26. January 2026. doi:10.12691/wjac-11-1-3

1. Introduction

The African crabwood, Carapa procera, is a species of the Meliaceae family, widely distributed in the wooded savannas of Africa and Latin America. It is a medicinal plant extensively used by rural populations, particularly in Mali and Senegal, for cosmetic and medicinal purposes for human and animal health [1] due to its rich and diverse biochemical composition of active compounds. Its oil, commonly known as touloucouna oil in West Africa, is extracted from the seeds (kernels) and is the most widely used part of the plant.

Carapa procera oil is distinguished by a complex and balanced chemical composition, combining unsaturated fatty acids, limonoids, triterpenes, and vitamins, making it a natural resource of great therapeutic, cosmetic, and industrial value that remains underutilized on a global scale. Among other minor compounds, it contains vitamins, alkaloids, and limonoids, which confer various properties such as medicinal, natural antioxidant, antibacterial, and antifungal effects [2]. This oil is also used in cosmetics, particularly for hair and skin care.

Although it is used in West Africa for these various purposes, the composition and physicochemical properties of african crabwood, oil which underlie its numerous biological properties are not yet well understood, especially in the specific case of plant oils from Senegal.

The objective of this study is to conduct a physical characterization of the seeds and determine the biochemical composition of Carapa procera oil specifically its composition of fatty acids, glycerides, sterols, tocopherols, and tocotrienols and to establish the link between these components and the oil’s applications. Knowledge of the chemical constituents in relation to the physicochemical properties described in the literature will make it possible to explore ways to utilize this oil.

2. Materials and Methods

2.1. Plant Material

The plant material used in this study consists of dry seeds of Carapa procera (Figure 1 a) from trees in the Ziguinchor region (12° 33’ 40’’ North, 16° 17’ 00’’ West) in southern Senegal. The average mass of the seeds was determined by weighing 50 seeds, and the average masses of the kernels (Figure 1 b) and husks (Figure 1 c) were determined after shelling these 50 seeds. The oil is extracted from the ground kernels (Figure 2).

Figure 1. Whole seeds (a), kernels (b) and hulls (c) of Carapa procera

Figure 2. Ground kernels from Carapa procera

2.2. Solvents and Reagents

All chemical reagents, standards and solvents were of the analytical type (HPLC grade), from Sigma-Aldrich, France.

2.3. Seeds Grinding

The raffinate is ground using a FRITSCH Pulverisette 19 knife mill equipped with screens having a mesh diameter of 1 mm.

2.4. Dry Matter

The dry matter (DM) content was determined according to French standard NF V 03-103. It corresponds to the mass loss undergone by a sample of about 1 g after drying in an oven at 103°C until a constant mass. The number of replicates analyzed is three (3) and the result is obtained by averaging the three (3) trials.

2.5. Lipids Content

The lipid contents were determined by using the standardized Soxhlet method (NF ISO 734-1) which consists of extracting the lipids contained in the matter with cyclohexane for minimum 6 hours. An amount of about 30 g of seeds was used. The Soxhlet extractor was equipped at its base with a 250 mL flask in which 200 mL of solvent were introduced. The oil used for the tocopherols analysis was extracted by cold centrifugation using cyclohexane. The number of replicates analyzed is three (3). The result is obtained by averaging the three (3) trials.

2.6. Fatty Acids Analysis

The fatty acids profile was determined by analysis of Fatty Acids Methyl Esters (FAME) in Gas Chromatography (GC) using Varian 3800 chromatograph according to the French Standard NF ISO 5508 standard. The esterification was carried out in two steps, solubilization of the oil by TBME (tert-butyl methyl ether) and uploading TMSH (trimethyl sulphonium hydroxide 0.5 M in methanol). The analysis was performed in type GC 3800 equipped with a Varian CP-select column for FAME fused silica WCOT (length 50 m, internal diameter 0.25 mm, film thickness 0.25 µm) coupled with a flame-ionization detector (FID) heating the components at 250°C. The carrier gas was helium (flow rate of 1 mL/min). The injection was Split (1: 100 µL 1 250°C for 55 min.). The temperature programming was 185°C for 40 min and then rises from 185°C to 250°C at 15°C/min and finally 250°C for 10.68 min (analysis time 55.01 min). The standard used was the MGFA (SI) and the data was processed with Varian Star software. The number of replicates analyzed is three (3) and the result is obtained by averaging the three (3) trials.

2.7. Glycerides and Triglycerides Analysis

Analysis of glycerides and triglycerides were carried out after the glycerides silylation by 50 µL of methyl imidazole with 1 mL MSHFBA (N-Methyl-N-trimethyl silyl-Hepta Fluoro butyramide). The analysis was performed by Gas Chromatography with a Perkin Elmer instrument equipped with a CP Sil column 8CB Low Bleed MS Varian, length 15 m, internal diameter 0.32 mm, film thickness 0.25 µm. The injection was on column type 1 µL. The temperature program was 55°C for 0.5 min, then 200°C/min to 340°C, 340°C for 40 min. Helium was the carrier gas (column head pressure 15 psi). The injection into the oven was performed under the following conditions: 55°C for 0.5 min, 45°C/min to 80°C, 10°C/min. up to 360°C and 360°C for 16 min. FID carried out detection at 365°C. The compounds were identified through comparison of the retention time with standards reference and the quantification was carried out by external calibration. The number of replicates analyzed is three (3) and the result is obtained by averaging the three (3) trials.

2.8. Phytosterols Analysis

Sterols were analyzed on the unsaponifiable fraction after silylation by MSHFBA (Methyl Trimethyl Silyl Hepta Fluoro ButyrAmide + 50 µL 1-methyl imidazole). The analysis was performed by Gas Chromatography (GC) with a Perkin Elmer instrument coupled to an coupled to an FID (365°C) and equipped with a column CPSil 8 CB (Varian) of length 30 m, diameter 0.25 mm and film thickness 0.25 µm. The injection was on column type (1µL), the carrier gas was helium and the column head pressure was 100 kPa. The injector temperature programming was 55°C for 0.5min, then increase from 55 to 340°C at 200°C/min and stabilization at 340°C for 30 min. The temperature of the oven was 160°C for 0.5 min, then rise from 160 to 260°C at 20°C/min and stabilization at 260°C for 5.5 min then rise from 260 to 300°C at 2°C/min then maintaining the temperature for 10 min at 300°C finally a rise from 300 to 350°C at 45°C/min and stabilization at 350°C for 3 min. The number of replicates analyzed is three (3). The result is obtained by averaging the three (3) trials.

2.9. Tocopherols and Tocotrienols Analysis

The analysis of the tocopherols of Africain crabwood seeds oil, using the α, β, γ and δ-tocopherols and δ-tocotrienol standards by external calibration was performed according to EN ISO 9936. Exactly 10 mg of oil were diluted with 1 mL of cyclohexane. The sample was analyzed by HPLC Dionex type equipped with a Kromasil 100 column SIL 5 µ (250 × 4 mm) and a fluorescence detector (λex = 290 nm and λem = 317 nm). The eluent was composed of mixture isooctane/isopropanol (99.5%/0.5%) at a flow rate of 1.1 mL/min. The number of replicates analyzed is three (3) and the result is obtained by averaging the three (3) trials.

3. Results and Discussion

3.1. Physical Characteristics of Carapa procera Seeds

The physical characteristics of the Carapa procera seed (Table 1) show that its average dry weight is 7.59 g. This seed is more than 27 times heavier than that of the neem (0.28 g) [3] and nearly 4 times heavier than that of the desert date palm (2.04 g) [4]. The proportions of the kernel (71.23%) and the shell (28.77%) indicate the kernel’s clear predominance in the seed. This kernel-to-shell ratio is similar to that of seeds such as the desert date palm. This kernel-to-shell ratio allows the seed to be processed without dehulling, even for an extraction process involving pressing, given the high average oil content in the kernel of 56.55%, noting that analysis confirms the shell contains no lipids. Although this content is high, it is slightly lower than that of African crabwood seeds from Benin, which is 61.5% [5]. It remains, however, higher than that of desert date kernels (34.5%), neem kernels (49%) [4], sunflower seeds (47.3%) [6], or Nigella seeds (13 to 40%) [7,8,9,10,11,12], but lower than that of Brazil nuts (Bertholletia myrtaceae) (72.5%) [13] and hazelnut seeds (62.4%) [14].

This oil is widely used in Senegal for various medicinal purposes. Carapa procera oil is very bitter; as is the case with neem oil [15], this bitterness may be due to molecules belonging to the triperpenoid family.

Table 1. Distribution of the kernel and hull in Carapa procera seeds

3.2. Composition of Carapa procera Seeds Oil

3.2.1. Fatty Acids Composition

The fatty acids composition of Carapa procera oil, determined by Gas Chromatography analysis (Figure 3), reveals the predominance of two (2) fatty acids: oleic acid C18:1 (45.70%) and palmitic acid C16:0 (32.75%), followed by linoleic acid C18:2 (13.02%) (Table 2). This fatty acid profile is similar to that obtained for seed oil from Togo, in which the predominant fatty acid is oleic acid (59.1%), followed by palmitic acid (21.1%), stearic acid (8.5%), and linoleic acid (8.1%).

Figure 3. Chromatogram of the fatty acid analysis

This fatty acid composition with the simultaneous presence of saturated palmitic and stearic acids, accounting for more than 39% of total fatty acids and more than 96.5% of saturated and unsaturated fatty acids, oleic and linoleic acids, of nearly 59%which accounts for nearly 99% of the unsaturated fatty acids confirms the unsaturated nature of this oil. The higher proportion of unsaturated fatty acids (59.39%), combined with a significant proportion of saturated fatty acids (40.61%), results in an unsaturated-to-saturated fatty acid ratio of approximately 2:3, indicating a certain balance, with unsaturated fatty acids predominating. This fatty acid profile is unique and not found in other vegetable oils. However, it shares certain similarities with neem oil, which also belongs to the Meliaceae family, particularly in terms of the predominance of the same fatty acids (oleic, linoleic, palmitic, and stearic), which account for 97.88% of the total fatty acids in Carapa oil and 95.8% in neem oil.

Table 2. Fatty acids composition (in %) of Carapa procera seeds oil

3.2.2. Glycerides and Triglycerides Composition

The results results obtained using GC (Figure 4) show that triglycerides account for 97.22% of this oil (Table 3). This triglyceride content is slightly lower than that of most seed oils and animal fats, which generally exceed 98%. [16]. The amounts of C16 and C18 diglycerides (2.07%) and C16 and C18 monoglycerides (0.23%) can be explained by the release of certain fatty acids resulting from the hydrolysis of the triglycerides in the oil, which could account for the decrease in the amount of triglycerides.. This results in the presence of free fatty acids (FFAs) (0.48%), which are responsible for the acidity of this oil.

Figure 4. Chromatogram of the analysis of glycerides and triglycerides

Table 3. Composition of glycerides in Carapa procera seeds oil

Analysis of the triglycerides shows that Carapa procera oil consists primarily of three major triglycerides: Palmitic-Linoleic-Stearic (PLS) (45.95%), followed by linoleic-palmitic-oleic and linoleic-stearic-linolenic (LPO+LSLl) (30.57%) and oleic-palmitic-palmitoleic and oleic-stearic-myristic (OPPl+OSM) (19.76%) (Table 4). This triglyceride profile is consistent with the oil’s fatty acid composition. This result confirms that this oil consists of storage lipids.

Table 4. Composition of glycerides in Carapa procera seeds oil

3.2.3. Phytosterols Content

Phytosterols, or plant sterols, are essential components of the unsaponifiable fraction of vegetable oils. In the case of Carapa procera oil, the analysis results of GC analysis (Figure 5) reveal a very high phytosterols content (9.09 g/kg of oil) (Table 5). This content is significantly higher than that of Carapa seed oil from Benin (2.49 g/kg) [5]. This very high phytosterols content makes Carapa procera oil from Casamance very rich in phytosterols. Its phytosterols content is far higher than that of neem oil (3.34 g/kg) [3] and desert date palm oil (2.11 g/kg) [15], peanut oil (1.6 g/kg), sunflower oil (4.3 g/kg), rapeseed oil (8.2 g/kg), corn germ oil (8.5 g/kg), and soybean oil (3.5 g/kg) [17,18] or black cumin seeds (1.4 to 2 g/kg) [19] all of which are known to be rich in phytosterols.

The oil from the seeds of African crabwood in southern Senegal is characterized by the presence of three (3) sterols: β-sitosterol, campesterol, and stigmasterol. It should be noted that these three phytosterols are abundant in nature [20]. β-sitosterol is by far the most abundant phytosterol, at 7.09 g/kg, accounting for 78% of the sterols in this oil. This β-sitosterol content (78%) is slightly higher than that of seeds from Benin (61.2%) [5]. It is followed by campesterol (1.47 g/kg, or 16.17%) and stigmasterol (0.53 g/kg, or 5.83%).

Figure 5. Chromatogram of the phytosterol analysis

β-sitosterol is the predominant component in many vegetable oils, occurring in varying proportions, such as in neem oil, desert date palm oil (35.54%), olive oil (84.3%), peanut oil (62.3%), sunflower oil (61.9%), canola oil (45–61%), soybean oil (47–59%), and sesame oil (59–62%) [21,22,23].

The high β-sitosterol content in Carapa procera oil is an interesting characteristic in that it contributes to the oil’s positive physiological effects on health [24]. In fact, numerous clinical studies have shown that consuming approximately 2 g of β-sitosterol per day lowers cholesterol by about 10% [25], and several scientific publications have focused on the antitumor effects of phytosterols, particularly β-sitosterol [26]. In fact, β-sitosterol is the most widely studied sterols due to its importance and its physiological effects on health [24]. Numerous clinical studies have shown that consuming approximately 2 g of β-sitosterol per day lowers cholesterol by about 10% [25].

It should be noted that, in general, phytosterols are of great importance due to their numerous biological properties (antioxidant, anti-polymerizing, and antimicrobial) and therapeutic properties (cholesterol-lowering, anti-inflammatory, anticancer, and anti-atherosclerotic) [19].

In fact, it has been shown that phytosterols can reduce the risk of certain types of cancer, including lung cancer [27], breast cancer [28], esophageal cancer [29], ovarian cancer [30], stomach cancer [31] and colon cancer [32]. It may also stimulate immune responses in people infected with HIV [33]. They protect Low-Density Lipoproteins (LDL) from free-radical-induced oxidation [34]. A growing body of research attributes the beneficial effects of phytosterols to their ability to reduce obesity [35] and has also demonstrated beneficial effects on Parkinson’s and Alzheimer’s diseases [36,37,38]. Phytosterols also play an important role in the pharmaceutical, nutritional, and cosmetic fields, particularly in the production of therapeutic steroids [20].

It should be noted that this very high content could be significantly reduced in oil obtained through artisanal or traditional methods of crushing Carapa procera seeds. This process involves roasting the kernels before grinding it and extracting the oil by cooking it with water.

Table 5. Phytosterols Composition of Carapa procera seeds oil

3.2.4. Composition of Tocopherols and Tocotrienols

The tocopherols content in Carapa procera oil obtained by HPLC analysis (Figure 6) is 25.87 mg/kg (Table 6). This content is lower than that of Benin seed oil (92 mg/kg). This result shows that the tocopherols and tocotrienols content is relatively low compared to that of several vegetable oils, such as desert date palm oil (512.40 mg/kg), neem oil (338.70 mg/kg) [15], sunflower oil (546 mg/kg), and canola oil (1153 mg/kg) [16]. This low tocopherols content in African crabwood seeds oil poses a challenge for its preservation, as tocopherols play an important role in stabilizing the oil during storage [39]. In fact, they prevent the oxidation of polyunsaturated fatty acids in the bloodstream and protect Low-Density Lipoproteins (LDL) from free-radical-induced oxidation, which leads to the development of atherosclerotic lesions [40,41].

However, it should be noted, that tocotrienols account for the majority of the tocopherols and tocotrienols in C. procera oil. In fact, with a concentration of 20.41 mg per 100 g of oil, tocotrienols make up 78.9% of the total tocopherols and tocotrienols. Bien que les teneurs en tocotriénols de l’huile palme soit bien plus élevée, leur proportion (70%) par rapport au total tocotriénols-tocophérols sont similaires, contrairement à la plupart des huiles végétales [42].

Gamma-tocotrienol is the predominant compound, at 12.67 mg/kg, accounting for 48.98% of total tocopherols and tocotrienols.

Figure 6. Chromatogram of the analysis of tocopherols and tocotrienols

This high proportion of tocotrienols in African crabwood oil is a mark of quality, which increases its value. Indeed, they possess numerous biological properties, particularly their positive effects on health. Tocotrienols are known for their role in bone resorption, diabetes [43], and cardiovascular diseases [43,44,45]. Tocotrienols also possess neuroprotective [46,47], anti-inflammatory [45,48], anticancer [43,45,46,48,49], dermo-cosmetic [50,51], cholesterol-lowering [46], lipid-lowering, and blood-sugar-lowering [45,46] properties. These numerous properties of tocotrienols, combined with those of phytosterols both present in significant amountsmake Carapa procera oil a product with great potential for commercial development.

Table 6. Tocopherols and tocotrienols content of Carapa procera seeds oil

4. Conclusion

This study made it possible to determine the composition of Carapa procera seeds oil harvested in Casamance. The kernel, which accounts for 71.23% of the seed, is the main source of oil, confirming the potential for commercial development of this still underutilized oilseed species.

The fatty acids profile, dominated by oleic acid (45.7%), followed by palmitic acid (32.75%) and linoleic acid (13.02%), falls within the ranges reported for this species in West and Central Africa, while exhibiting a relatively high oleic acid content. This balance between monounsaturated (59.39%) and saturated (40.6%) fatty acids gives the oil good oxidative stability while maintaining a significant content of the essential fatty acid (linoleic acid).

Triglycerides analysis reveals an oil composed almost entirely of triglycerides (97.22%), dominated by T52 triglycerides primarily palmitic-linoleic-stearic (nearly 46%) and T54 triglycerides, consisting of linoleic-palmitic-oleic and linoleic-stearic-linolenic (approximately 30.5%), with smaller proportions of T50, composed of oleic-palmitic-palmitoleic and oleic-stearic-myristic (approximately 20%), T56 (2.57%), and T48 and T58 (<1% each). This distribution directly reflects the combination of the major identified fatty acids and indicates an oil with a homogeneous triglyceride composition, consistent with the fatty acid profile.

In terms of minor bioactive compounds, the oil contains a significant amount of phytosterols (9.09 g/kg), dominated overwhelmingly by β-sitosterol (78%) and campesterol (16.17%), compounds known for their cholesterol-lowering and anti-inflammatory properties. The tocopherol fraction (25.87 mg/kg), mean while, is unique in its high content of γ-tocotrienol (approximately 49%), α-tocotrienol (17.2%), δ-tocotrienol (approximately 12.72%) rather than conventional tocopherols, with α-tocopherol accounting for only 15.73% of the total. This predominance of tocotrienols molecules with strong antioxidant and neuroprotective potential distinguishes this oil from common vegetable oils and enhances its nutraceutical and cosmetic value.

Overall, Carapa procera seeds oil from Casamance (southern Senegal) appears to be a fat with a balanced lipid profile, rich in minor bioactive compounds (phytosterols and tocotrienols), which supports its traditional uses (skin care, therapeutic applications) and opens up opportunities for its commercialization in the cosmetic, pharmaceutical, and nutraceutical sectors. Further research on oxidative stability, in vitro antioxidant activity, and the characterization of limonoids would help complete this characterization and better position this oil among unconventional African oils.

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