@article{plant2017515,
author={{Islam, A. T. M. Rafiqul and Islam, Md. Monirul and Alam, M. Firoz},
title={Rapid <i>in</i><i> vitro</i> Clonal Propagation of Herbal Spice, <i>Mentha piperita </i>L. Using Shoot Tip and Nodal Explants},
journal={Research in Plant Sciences},
volume={5},
number={1},
pages={43--50},
year={2017},
url={http://pubs.sciepub.com/plant/5/1/5},
issn={2333-8539},
abstract={A high frequency efficient protocol for rapid propagation of the herbal spice <i>Mentha piperita</i> L. from shoot tip and nodal explants was established by using full and half strength of Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzyl amino purine (BAP; 1.0-5.0 mg/L) and kinetin (Kn; 1.0-5.0 mg/L). The highest number of shoots (42.0) with 100% frequency was obtained from nodal explants in the full strength of medium containing 3.0 mg/L BAP. For further elongation, microshoots were transferred to MS medium containing different concentrations of gibberellic acid (GA<SUB>3</SUB>; 0.5-2.0 mg/L). The highest shoot length (13.1 cm) with 100% frequency was achieved on medium containing 1.0 mg/L GA<SUB>3</SUB>. <i>In vitro</i> proliferated shoots were then excised from the shoot clumps and transferred to the rooting medium containing different concentrations of indole butyric acid (IBA; 0.5-2.0 mg/L) and indole acetic acid (IAA; 0.5-2.0 mg/L) alone. Among these, the highest root proliferation was obtained in the medium containing 1.5 mg/L IBA. The rooted plantlets were hardened on MS basal liquid medium and subsequently in polycups containing sterile soil and vermiculite (1:1) and finally transferred to the field. The survival rate was 100% after 25 days.},
doi={10.12691/plant-5-1-5}
publisher={Science and Education Publishing}
}