eng Science and Education Publishing Research in Plant Sciences 2333-8539 2015-04-09 3 1 18 26 10.12691/plant-3-1-4 PLANT2015314 article Ntsomboh-Ntsefong Godswill ntsomboh@yahoo.fr 1 2 Epoh-Nguea Toussaint 3 Madi- Galdima 3 Nsimi-Mva Armand 4 4 Ngando-Ebongue Georges Frank 4 Kounga Tagne Samuel 4 Mpondo Mpondo Emmanuel 4 Dibong Didier 5 Institute of Agricultural Research for Development (IRAD), Specialized Centre for Oil Palm Research of La Dibamba (CEREPAH), P. O. Box 243 Douala, Cameroon Faculty of medicine and Pharmaceutical sciences (FMSP), Department of Pharmacy-Ethno pharmacology and Applied Botany, University of Douala, Cameroon Institute of Agricultural Research for Development (IRAD), S. W. Regional Centre, Ekona-Cameroon Department of Plant Biology, Faculty of Science, B. P. 24157, University of Douala, Cameroon Vascular wilt disease caused by the fungus Fusarium oxysporum f. sp. elaeidis (F.o.e) is one of the major diseases of oil palm (Elaeis guineensis Jacq.) in the South-West region of Cameroon. In order to document the F.o.e isolation and characterization procedures, a 3 months study was carried out in palm plantations around IRAD Ekona and in the palm pathology laboratory located in the South-West region of Cameroon. Some 30 isolates of F.o.e and 10 g of infected soil were collected on and around infected oil palm trees from the field and used to appreciate various morphological characters that influence their aptitude to persist in the soil and on the host. The in vitro mycelial growth, sporulation (conidia), growth rate, biomass and the other characteristics as well as the correlation of the isolates in the culture media were recorded. Results showed that the isolates develop very quickly in the Medium for Mycelium (MM) contrary to PDA medium. In MM, the conidial stocks developed and filled the Petri dish in about 3 days contrary to the filamentous fungal stocks which took about 7 days to fill the Petri dish. These results can improve understanding of the procedures used and devise a selection strategy of the antagonistic microbial stocks for a biological control against oil palm vascular wilt disease. http://pubs.sciepub.com/plant/3/1/4/plant-3-1-4.pdf Fusarium oxysporum f sp elaeidis oil palm vascular wilt Elaeis guineensis Jacq in vitro characterization