@article{plant2013111,
author={AUTHOR = {Akbari, Mohammad and Nadaf, Esmaeil and Lotfi, Mahmoud and Tohidfar, Masoud},
title={Transformation of Iranian Melon for Increasing Resistance to Fungal Diseases},
journal={Research in Plant Sciences},
volume={1},
number={1},
pages={1--3},
year={2013},
url={http://pubs.sciepub.com/plant/1/1/1},
abstract={Melon (<i>Cucumis</i> <i>melo</i> L.) is one of the most important horticultural crops that fungal diseases are one of factors limiting its production. So, resistant cultivars have particular importance. <i>Chitinase</i> and <i>¦Â-</i><i>Glucanase</i> are well-known enzymes for increasing plant resistance to fungal pathogens. Thereby, transformation of Khatooni cultivar as the most important melon in Iran was carried out. Gene constructs consisting of the <i>chitinase</i>, <i>¦Â-1, 3 Glucanase</i>, a selector gene (ntpII) and cauliflower mosaic virus (CaMV) 35S promoter were transferred by <i>Agrobacterium</i>. Transgenic plants were regenerated from medium containing kanamycin (25mg/l) cefotaxime (300mg/l) and BAP (1mg/l). The presences of gene constructs were proved by PCR using specific primers. In this study, the efficacy of transgenic shoots in Khatooni cultivar was 2.7 percent.},
doi={10.12691/plant-1-1-1}
publisher={Science and Education Publishing}
}