@article{jpm2013121,
author={{Ennamany, Rachid and Leconte, Nadine and Lecrlerc, Jacques and Jab¨¨s, Aliza and Rezvani, Hamid-Reza and Rambert, J¨¦r?me and Ezzedine, Khaled and Mossalayi, M. Djavad},
title={Sublethal UVB Induces DNA Lesions and Pro-Apoptotic Gene Transcription in Human Keratinocytes: Attenuation by a Mixture of Plant Extracts},
journal={World Journal of Preventive Medicine},
volume={1},
number={2},
pages={4--10},
year={2013},
url={http://pubs.sciepub.com/jpm/1/2/1},
abstract={Ultraviolet B (UVB) radiation is a potent environmental factor in skin pathogenesis as it modulates oxidative burst, gene expression and proliferation/apoptosis of human normal keratinocytes. In the absence of cell apoptosis, sublethal UVB is shown to induce significant increase of mitochondrial and nuclear DNA lesions as quantified by increased apurinic/apyrimidinic sites together with transcriptomic modulation of pro-apoptosis/survival genes. These effects may be attenuated by cell pretreatment with extracts from three various plants: <i>Papaver rhoeas, Passiflora incarnata and Anchusa arvensis </i>or their mixture. Extracts variably decreased UVB-induced mitochondrial and nuclear DNA lesions in normal human keratincoytes. When mixed together, higher reduction of DNA lesions has been observed at lower extracts¡¯ concentrations, compared to each extract alone which suggested their synergy. Furthermore, sublethal UVB-induced transcriptomic modulation was significantly prevented by cell preincubation with extract mixture through coordinated reduction of pro-apoptotic gene transcription while increasing pro-survival related transcripts. Finally, extract mixture protected keratinocytes against lethal UVB dose. This work reveals sublethal UVB-mediated lesions at both DNA and RNA levels in human keratinocytes and the ability of appropriate plant-derived sunscreen to reduce cell injury.},
doi={10.12691/jpm-1-2-1}
publisher={Science and Education Publishing}
}