@article{jfs20221031,
author={{N¡¯Zi, N¡¯Goran Parfait and Ouattara, Hadja Dj¨¦n¨¦ba and Attien, Yao Paul and Gbonon, Val¨¦rie Carole and Guessennd, Nathalie Kouadio and Angaman, Dj¨¦doux Maxime},
title={Characterization of Psychrophilic Pathogens in Ready-to-eat Salads Sold in Supermarkets in the City of Abidjan (C?te d¡¯Ivoire)},
journal={Journal of Food Security},
volume={10},
number={3},
pages={89--96},
year={2022},
url={http://pubs.sciepub.com/jfs/10/3/1},
issn={2372-0107},
abstract={Ready-to-eat salads have become highly demanded foods in the city of Abidjan (C?te d¡¯ivoire) because of their considerable benefits both nutritionally and therapeutically. However, these foods are considered products that are very vulnerable to microbial contamination. In order to contribute to food safety in C?te d'Ivoire, in particular among the Abidjan population, we have set the objective of inventorying psychrophilic pathogenic species in ready-to-eat salads sold in supermarkets. Thus, a total of 60 samples were analyzed using the techniques of classical microbiology, molecular biology, bioinformatics and statistics. For the search for psychrophilic pathogens, the selective culture medium (Oxford agar) of the pathogens frequently isolated (<i>L. monocytogenes</i>) was used. Microbiological analyzes revealed the presence of similar germs in 6 samples based on growth characters with Oxford¡¯s selective medium. However, molecular sequencing allowed to identify six (6) different pathogens, <i>Lysinibacillus fusiformis</i> in spinach salads (EP), <i>Listeria monocytogenes</i> in mixed salads (SCOM), <i>Staphylococcus saprophyticus</i> in lamb's lettuce and arugula, <i>Staphylococcus fleurettii</i> in carrots (SCA), <i>Staphylococcus nepalensis</i> in young shoots (JP) and <i>Bacillus cereus</i> in cabbage (SCH). The growth kinetics showed the characterized species grow significantly in the different lettuces under domestic storage conditions after opening the packaging except for <i>S. fleurettii.</i> However, the growth of <i>L. fusiformis</i> and <i>B. cereus</i> was found to be more significant. In conclusion, <i>S. saprophyticus</i>,<i> S. nepalensis,</i> <i>B. cereus</i>, <i>L. fusiformis</i> and <i>B. cereus</i> grow easily on oxford agar presenting similar culture characters like <i>L. monocytogenes</i>. In addition, the presence of unknown pathogenic species in ready-to-eat salads and their significant growth during the domestic storage period is a real public health problem. It would therefore be wise for the health authorities to set up a monitoring program for these species in the ready-to-eat sold in supermarkets in C?te d'Ivoire.},
doi={10.12691/jfs-10-3-1}
publisher={Science and Education Publishing}
}