eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2021-09-22 9 9 477 483 10.12691/jfnr-9-9-4 JFNR2021994 article Tito Naeem Habib 1 Gamal El-Din S. Amin 1 Ahmed Ahmed Hussien 1 Mohammed Salama 2 Mohamed Omar Ahmed 2 Motamed Elasyed Mahmoud motamed71111@gmail.com, moatamed_ali@vet.sohag.edu.eg 3 Molecular Genetics Lab, Department of Zoology, Faculty of Science, Sohag University, 82524, Egypt Department of Zoology, Faculty of Science, Ain Shams University, 11566, Egypt Department of Animal Behavior and Husbandry (Genetics, Breeding and Production), Faculty of Veterinary Medicine, Sohag University, 82524, Egypt The abuse of contraceptive pills as growth promoters in the poultry industry has serious health concerns. Consumption of hormonal residues in animal products results in reproductive disorders and an increased risk of cancers in humans. This study aimed to investigate the effect of treatment with progesterone hormone on the plasma proteins and RAPD-DNA markers of broiler chickens. One hundred, one-day-old broiler chicks were equally divided into 5 groups (n = 20). Four groups were treated daily with progesterone hormone either subcutaneously or orally (1.0 mg, 1.5mg/kg). The 5th group was control non-treated chicken. Sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE), and RAPD assays in conjunction with productive parameters such as growth rate were assessed. SDS-PAGE and RAPD-DNA patterns were significantly affected by progesterone hormone treatment. Either or oral or subcutaneous progesterone hormone treatments increased the body weight of chicks compared to control. Treatment with progesterone hormone induces effects on both DNA and plasma proteins in broiler chickens. Changes at the genomic DNA level may be due to specific modifications in RAPD patterns or arise as a consequence of hot spot DNA changes and/or mutations. http://pubs.sciepub.com/jfnr/9/9/4/jfnr-9-9-4.pdf Xeno-progesterone plasma protein profile RAPD-DNA markers growth rate broiler chicken