eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2021-05-05 9 5 240 250 10.12691/jfnr-9-5-1 JFNR2021951 article Leong-Perng Chan 1 2 Ya-Ping Tseng 3 Cheng Liu orangeliu@gmail.com; tinna_ling@mail.cnu.edu.tw 4 5 6 Chia-Hua Liang orangeliu@gmail.com; tinna_ling@mail.cnu.edu.tw 7 Department of Otorhinolaryngology-Head and Neck Surgery, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan Department of Health and Beauty, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan Department of Cosmetic Science and Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan Fermented foods contain beneficial probiotics, which thus results in products with improved nutritional properties and healthy effects. There is however a need for research on clinical trials to measure the effects of fermented foods in clinical applications and in skincare. The composition of fermented vegetable-fruit drink (FVFD) included the fermented vegetable-fruit juice, apple juice, fructose, high methoxyl pectin, citric acid, apple flavor and water. In this study, the effects of FVFD on the free radical scavenging activities, promotion of skin cell proliferation and collagen synthesis were investigated. A randomized, controlled, double-blind study design was used to assess FVFD and basic drink (50 ml of a FVFD/placebo drink daily for 8 weeks for each subject). Thirty subjects (aged 35-55 years old) were recruited for an 8 week-long clinical trial to confirm the efficacy of FVFD in improving the serum biochemical superoxide dismutase (SOD) and catalase levels, and anti-skin age markers, including improving skin moisture, brightness and elasticity, reducing crow¡¯s feet, skin texture, wrinkles, pores and spots, and increasing amount of skin collagen. The results suggest that fermented vegetable-fruit drink has anti-oxidant activities, promotes collagen synthesis and delays the aging of skin with potential applications in food additives. http://pubs.sciepub.com/jfnr/9/5/1/jfnr-9-5-1.pdf fermented vegetable-fruit drink skin anti-oxidant anti-aging