@article{jfnr2021933,
author={{Bursal, Ercan and Y?lmaz, Mustafa Abdullah and Aras, Abd¨¹lmelik and T¨¹rkan, Fikret and Yildiko, ¨¹mit and K?l??, ?mer and Dey, Abhijit},
title={Determination of Phenolic Content, Biological Activity, and Enzyme Inhibitory Properties with Molecular Docking Studies of <i>Rumex nepalensis</i>, an Endemic Medicinal Plant},
journal={Journal of Food and Nutrition Research},
volume={9},
number={3},
pages={114--123},
year={2021},
url={http://pubs.sciepub.com/jfnr/9/3/3},
issn={2333-1240},
abstract={This study was conducted to evaluate the phenolic content, antioxidant potential, and enzyme inhibitiory properties of <i>Rumex </i><i>nepalensis</i><i> </i>by <i>in vitro</i> spectrophotometric methods. The experiments demonstrated that glutathione S-transferase (GST), ¦Á-glycosidase (¦Á-Gly), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzymes were strongly inhibited by <i>R. </i><i>nepalensis</i><i> </i>extracts. The IC<SUB>50</SUB> values for GST, ¦Á-Gly, AChE, and BChE enzyme inhibitions were calculated as 21.01 mg/mL, 34.65 mg/mL, 27.72 mg/mL, and 17.32 mg/mL, respectively. Also, effective antioxidant capacities of water and methanol extracts of <i>R. </i><i>nepalensis</i> were determined by ABTS, CUPRAC, DPPH, and FRAP methods. Furthermore, quinic acid (15.61 mg/g), miquelianin (2.06 mg/g), quercitrin (1.97 mg/g), and protocatechuic acid (0.217 mg/g) were identified to be the major phenolic compounds of the plant extract according to the LC-MS/MS analysis. Finally, molecular docking studies were carried out to show the interactions of quinic acid, miquelianin, and quercitrin with AChE, BChE, GST, and ¦Á-Gly enzymes. Docking analysis indicated the possible roles of these phytochemicals in enzyme inhibitory activities.},
doi={10.12691/jfnr-9-3-3}
publisher={Science and Education Publishing}
}