eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2021-02-05 9 2 73 78 10.12691/jfnr-9-2-3 JFNR2021923 article Yung-Kai Lin 1 Wei-Chun Hu 2 Chi-Fu Chiang dofugood1205@outlook.com 3 Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung, Taiwan Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan Many Chinese herbal medicines have been proven to have anti-inflammatory effects. Among them, edible and medicinal mushrooms are rich in a variety of physiologically active ingredients, which can enhance immunity and are good materials for the development of health foods. This article aims to investigate the beneficial effects of SOD Light, a herbal formula consisting of Sparassis crispa, Aureobasidium pullulan, and Ganoderma lucidum, on anti-inflammation, antioxidant, and immune-modulation. SOD Light was able to inhibit ROS expression level and boost the SOD activity in PBMCs by 11% and 43.8%, respectively, as compared with the control group, and the significant improvement effect of the phagocytic activity of neutrophils was exhibited. Moreover, SOD Light also significantly enhanced the expression of the lymphocyte development-associated and adaptive immune-related genes (i.e., CD134, CD26, CD96, CD247, and IL-16). This study successfully unveils that the combination of S. crispa, A. pullulans, and G. lucidum was available for anti-oxidation, cytokines production, enhancement of T cell activation, and lymphocyte development in PBMCs, which implies that SOD Light are beneficial for immune modulation. http://pubs.sciepub.com/jfnr/9/2/3/jfnr-9-2-3.pdf antioxidant Aureobasidium pullulan Ganoderma lucidum Sparassis crispa immune modulation