eng Science and Education Publishing Journal of Food and Nutrition Research 2333-1240 2021-01-31 9 2 61 67 10.12691/jfnr-9-2-1 JFNR2021921 article Ma Funing 1 Tariq Masood sss1984006@163.com, tariqafridi@aup.edu.pk 1 2 Huang Dongmei 2 Wu Bin 2 Ge Yu 2 Chen Di 2 Gutierrez-Pajares Jorge L 2 N Nasiruddin 3 Song Shun sss1984006@163.com, tariqafridi@aup.edu.pk 3 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Hainan 571101, China Direcotrate General Agricultural Research, Khyber Pakhtunkhwa, Peshawar, Pakistan In this research, we investigated the inhibitory effect of the ethanolic (EtOH) extract of bignay (Antidesma bunius) fruit on the viability of breast cancer cells (MDA-MB-231) by CCK-8 assay. The results showed that the half inhibitory concentration (IC50) was 219 ¦Ìg/mL after the cells were treated with EtOH extract for 72 hours. The EtOH extract was sequentially extracted by petroleum ether (PET), ethyl acetate (EtOAc), and n-butanol (nBuOH). Among them, the EtOAc fraction showed higher anti-proliferative activity than the other fractions. Wound healing experiment showed the EtOAc fraction inhibited the migration of MDA-MB-231 cells and significantly delayed the transition of the G1 to S phase compared to control. UHPLC-MS/MS analysis showed that in addition to citric acid and phenolic acids (3-Hydroxybenzoic acid, aspirin, fertaric acid), EtOH extract of bignay fruit contains active ingredient like coumarin, genistin, amentoflavone, and luteolin 7-galactoside. The anti-proliferation activity of amentoflavone against MDA-MB-231 cells was also confirmed. http://pubs.sciepub.com/jfnr/9/2/1/jfnr-9-2-1.pdf breast cancer ethno-medicine flavonoid tropical fruits