@article{jfnr2021921,
author={{Funing, Ma and Masood, Tariq and Dongmei, Huang and Bin, Wu and Yu, Ge and Di, Chen and L, Gutierrez-Pajares Jorge and Nasiruddin, N and Shun, Song},
title={Inhibitory Activity of Fruits Extracts of <i>A</i><i>n</i><i>t</i><i>i</i><i>d</i><i>e</i><i>s</i><i>m</i><i>a </i><i>b</i><i>unius</i><i> </i>on the Proliferation and Migration of MDA-MB-231 Breast Cancer Cells},
journal={Journal of Food and Nutrition Research},
volume={9},
number={2},
pages={61--67},
year={2021},
url={http://pubs.sciepub.com/jfnr/9/2/1},
issn={2333-1240},
abstract={In this research, we investigated the inhibitory effect of the ethanolic (EtOH) extract of bignay (<i>Antidesma bunius</i>) fruit on the viability of breast cancer cells (MDA-MB-231) by CCK-8 assay. The results showed that the half inhibitory concentration (IC50) was 219 ¦Ìg/mL after the cells were treated with EtOH extract for 72 hours. The EtOH extract was sequentially extracted by petroleum ether (PET), ethyl acetate (EtOAc), and n-butanol (nBuOH). Among them, the EtOAc fraction showed higher anti-proliferative activity than the other fractions. Wound healing experiment showed the EtOAc fraction inhibited the migration of MDA-MB-231 cells and significantly delayed the transition of the G1 to S phase compared to control. UHPLC-MS/MS analysis showed that in addition to citric acid and phenolic acids (3-Hydroxybenzoic acid, aspirin, fertaric acid), EtOH extract of bignay fruit contains active ingredient like coumarin, genistin, amentoflavone, and luteolin 7-galactoside. The anti-proliferation activity of amentoflavone against MDA-MB-231 cells was also confirmed.},
doi={10.12691/jfnr-9-2-1}
publisher={Science and Education Publishing}
}