@article{jfnr20219128,
author={{Jan, Bisma and Zahiruddin, Sultan and Basist, Parakh and Khan, Mohammad Umar and Abass, Sageer and Dewangan, Rikeshwer Prasad and Ahmad, Sayeed},
title={Separation and Identification of Antioxidant and Antihyperglycemic Constituents from <i>Morus alba</i> Infusion by TLC-MS-Bioautography and UPLC-MS},
journal={Journal of Food and Nutrition Research},
volume={9},
number={12},
pages={670--690},
year={2021},
url={http://pubs.sciepub.com/jfnr/9/12/8},
issn={2333-1240},
abstract={<i>Morus alba (M. alba)</i> popularly known as white mulberry is a well-known and globally utilized functional food and nutraceutical. The effect of different brewing time (BT) on antioxidant and antihyperglycemic compounds released from <i>M. alba</i> leaves infusion was studied. Major compounds responsible for antioxidant activity were identified and quantified by high-performance thin-layer chromatography (HPTLC). Additionally, thin layer chromatography-mass spectrometry-bioautography (TLC-MS-bioautography) analysis was performed to identify the compounds responsible for antioxidant and antihyperglycemic activity. <i>In vitro </i>¦Á-amylase and ¦Á-glucosidase inhibitory activity grew in a linear manner with each BTs. Based on the findings, 7 and 10 minutes of BTs at 98¡ãC were chosen as optimal for infusion preparation. Major phenolic acid i.e. chlorogenic acid at 7 and10 minutes of BT in <i>M. alba</i> infusion was quantified as 89.93 ¦Ìg/mL and 95.0 ¦Ìg/mL respectively. However, major flavonoid rutin was calculated as 5.56 ¦Ìg/mL and 8.79 ¦Ìg/mL respectively. Deoxynojirimycin (DNJ), which is a potent ¦Á-glucosidase inhibitor was identified in <i>M. alba</i> infusion by ultra performance liquid chromatography-mass spectrometry (UPLC-MS) at Rt of 0.88 minutes. TLC-MS-bioautography revealed that chlorogenic acid, rutin, and DNJ are predominant antioxidant and antihyperglycemic compounds in <i>M. alba</i> infusion. UPLC-MS fingerprinting analysis concluded that <i>M. alba</i> infusion at 98¡ãC for 7 and 10 minutes may be a good source of important alkaloids (fagomine), flavonoids (quercetin, morin), and carboxylic acid (fumaric acid), and other metabolites responsible for its antioxidant and antihyperglycemic activity favoring its utilization as a functional drink with therapeutic potential.},
doi={10.12691/jfnr-9-12-8}
publisher={Science and Education Publishing}
}