@article{jfnr20219122,
author={{Yang, Yuanyuan and Wang, Songmao and Yu, Kechen and Xu, Shiyi and Liu, Mengzhu and Zheng, Jianyong and Yuan, Wei},
title={Metabolic Engineering of <i>Escherichia coli</i> for Production of Ectoine},
journal={Journal of Food and Nutrition Research},
volume={9},
number={12},
pages={626--632},
year={2021},
url={http://pubs.sciepub.com/jfnr/9/12/2},
issn={2333-1240},
abstract={Ectoine, a cyclic amino acid derivative is produced by some moderately halophilic microorganisms. It is widely used in the field of medicine and cosmetics; however its high cost, low yield and complicated production process restrict its use in an industry. The present study was carried out to construct an engineered <i>Escherichia coli</i> for the heterologous ectoine production. Firstly, the ectoine synthesis cluster (<i>ectABC</i> genes) from <i>Halomonas elongata</i> were introduced into <i>E. coli</i> for the ectoine production in heterologous system. Secondly,<i> </i>the effect of deletion for<i> lysA</i> and <i>thrA</i> on producing ectoine was investigated. Thirdly, the bioconversion conditions for synthesizing ectoine were optimized. In this study, nine strains of <i>E.coli</i> BW25113 were constructed, and an engineering strain ABC (?<i>lysA</i>)/BW25113 with ectoine synthesis ability was screened out. An improved yield of ectoine was obtained with optimized conditions like 100 mmol/L sodium aspartate, 100 mmol/L glycerol, 80 mmol/L glucose and 120 mmol/L KCl at temperature 30ˇăC, and the pH 7.0. A yield 2.08 g/L of ectoine was obtained. After preliminary optimization, the ABC (?<i>lysA</i>)/BW25113 catalytic synthesis of ectoine increased by 2 times, indicating that optimized reaction conditions can increase the yield of ectoine. Here, we successfully constructed an engineered<i> Escherichia coli</i> for the heterologous production of ectoine. Our study herein provides a feasible and valuable biosynthesis pathway of ectoine with a potential for large-scale industrial production using simple and cheap feedstocks.},
doi={10.12691/jfnr-9-12-2}
publisher={Science and Education Publishing}
}